DNA Fingerprinting of Cannabis sativa Using Inter-Simple Sequence Repeat (ISSR) Amplification

Mareshige Kojoma; Osamu Iida; Yukiko Makino; Setsuko Sekita; Motoyoshi Satake

doi:10.1055/s-2002-19875

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Planta Med 2002; 68(1): 60-63
DOI: 10.1055/s-2002-19875

Original Paper

Analysis
© Georg Thieme Verlag Stuttgart · New York

DNA Fingerprinting of Cannabis sativa Using Inter-Simple Sequence Repeat (ISSR) Amplification

Mareshige Kojoma^1, ⁴ , Osamu Iida¹ , Yukiko Makino² , Setsuko Sekita³ , Motoyoshi Satake³

¹Izu Experimental Station for Medicinal Plants, National Institute of Health Sciences, Japan
²Kanto-Shin'etsu Regional Narcotic Control Office, Japan
³Division of Pharmacognosy and Phytochemistry, National Institute of Health Sciences, Tokyo, Japan
⁴Plant Functions Laboratory, RIKEN (The Institute of Physical and Chemical Research), Saitama, Japan

Further Information

Publication History

January 19, 2001

June 9, 2001

Publication Date:
31 January 2002 (online)

Also available at

Abstract
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Abstract

Chemical analysis of cannabinoid, and Inter-Simple Sequence Repeat (ISSR) fingerprinting of DNA were used to identify different samples of Cannabis sativa L. for forensic purposes. Three samples were classified into two types, tetrahydrocannabinol (THC) and cannabidiol (CBD) chemo-types, by high performance liquid chromatography (HPLC). The two samples of the CBD type were not distinguished by their HPLC patterns. ISSR fingerprinting identified polymorphic DNA patterns between these samples. ISSR fingerprinting clearly differentiated between cannabis samples that could not be achieved by HPLC analysis.

Key words

Cannabis sativa - hemp - Cannabaceae - DNA profiling - ISSR - cannabinoid - HPLC

References

1 Quimby M W, Doorenbos N J, Turner C E, Masoud A. Mississippi-grown marihuana-Cannabis sativa cultivation and observed morphological variations. Economic Botany. 1973; 27 117-27

PubMed Search in Google Scholar
2 Small E, Beckstead H D, Chan A. The evolution of cannabinoid phenotypes in Cannabis . Economic Botany. 1975; 29 219-32

PubMed Search in Google Scholar
3 Small E, Cronquist A. A practical and natural taxonomy for Cannabis . Taxon. 1976; 25 405-35

PubMed Search in Google Scholar
4 Fetterman P S, Keith E S, Waller C W, Guerrero O, Doorenbos N J, Qinby M W. Mississippi-grown Cannabis sativa L.: preliminary observation on chemical definition of phenotype and variations in tetrahydrocannabinol content versus age, sex, and plant part. Journal of Pharmaceutical Sciences. 1971; 60 1246-9

PubMed Search in Google Scholar
5 Rowan M G, Fairbairn J W. Cannabinoid patterns in seedling of Cannabis sativa L. and their use in the determination of chemical race. Journal of Pharmaceutical Pharmacology. 1977; 29 491-4

PubMed Search in Google Scholar
6 Baker P B, Gough T A, Taylor B J. The physical and chemical features of Cannabis plants grown in the United Kingdom of Great Britain and Northern Ireland from seeds of known origin. Bulletin on Narcotics. 1982; 34 27-36

PubMed Search in Google Scholar
7 Gillian R, Cole M D, Linacre A, Thorpe J W, Watson N D. Comparison of Cannabis sativa by random amplification of polymorphic DNA (RAPD) and HPLC of cannabinoid: a preliminary study. Science & Justice. 1995; 35 169-77

PubMed Search in Google Scholar
8 Jagadish V, Robertson J, Gibbs A. RAPD analysis distinguished Cannabis sativa samples from different sources. Forensic Science International. 1996; 79 113-21

Crossref PubMed Search in Google Scholar
9 Gigliano G S, Finizio A D, Caputo P, Cozzolino S. Cannabis fingerprints by using Random Amplified Polymorphic DNA (RAPD). Delpinoa. 1995 - 6; 37 - 8 35-47

PubMed Search in Google Scholar
10 Shirota O, Watanabe A, Yamazaki M, Saito K, Shibano K, Sekita S, Satake M. Random amplified polymorphic DNA and restriction fragemnt length polymorphism analyses of Cannabis sativa . Natural Medicines. 1988; 52 160-6

PubMed Search in Google Scholar
11 Gigliano G S. Restriction profiles of trnL (UAA) intron as a tool in Cannabis sativa L. identification. Delpinoa. 1995 - 6; 37 - 8 85-95

PubMed Search in Google Scholar
12 Gigliano G S, Caputo P. Ribosomal DNA analysis as a tool for the identification of Cannabis sativa L. specimens of forensic interest. Science & Justice. 1997; 37 171-4

PubMed Search in Google Scholar
13 Gigliano G S, Finizio A D. The Cannabis sativa L. fingerprint as a tool in forensic investigations. In: Narcotics, vols. XLIX and L, Nos. 1 and 2. 1997/1998: 129-37

Search in Google Scholar
14 Gigliano G S. Identification of Cannabis sativa L. (Cannabaceae) using restriction profiles of the internal transcribed spacer II (IRS2). Science & Justice. 1998; 38 225-30

PubMed Search in Google Scholar
15 Gigliano G S. Preliminary data on the usefulness of internal transcribed spacer I (ITS1) sequence in Cannabis sativa L. identification. Journal of Forensic Sciences. 1999; 44 475-7

PubMed Search in Google Scholar
16 Miyahara M, Sugaya K, Tanimura A, Satake M. Nucleotide sequences of 5S-rRNA gene spacer region from Moraceae and Cannabaceae. Natural Medicines. 1998; 52 209-12

PubMed Search in Google Scholar
17 Kohjyouma M, Lee I, Iida O, Kurihara K, Yamada K, Makino Y, Sekita S, Satake M. Intraspecific variation in Cannabis sativa L. based on intergenic spacer region of chloroplast DNA. Phar. Bull. Biological & Pharmaceutical Bulletin. 2000; 23 727-30

PubMed Search in Google Scholar
18 Prevost A, Wilkinson M J. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical Applied Genetics. 1999; 98 107-12

Crossref PubMed Search in Google Scholar
19 Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceeding of the National Academy of Sciences of the USA. 1979; 76 5269-73

PubMed Search in Google Scholar
20 Sneath P HA, Sokal R R. In: Numberical taxonomy. San Francisco; Freeman 1973

Search in Google Scholar

Mareshige Kojoma

Plant Functions Laboratory

RIKEN (The Institute of Physical and Chemical Research)

2-1 Hirosawa

Wako-shi

Saitama 351-0198

Japan

Fax: +81-48-467-5407

Email: kojoma@postman.riken.go.jp