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Plant Biol (Stuttg) 2002; 4(1): 46-52
DOI: 10.1055/s-2002-20435
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Callose in Frankia-Infected Tissue of Datisca glomerata is an Artifact of Specimen Preparation[*]

K. R. Jacobsen 1,2 , A. M. Berry 3
  • 1 Department of Agronomy and Range Science, University of California, Davis, CA 95616, USA
  • 2 Present address: Department of Botany, University of Wisconsin, Madison, WI 53706, USA
  • 3 Department of Environmental Horticulture, University of California, Davis, CA 95616, USA
Further Information

Publication History

June 18, 2001

January 02, 2002

Publication Date:
28 February 2002 (online)

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Abstract

Callose, or β-1,3-glucan, is a plant cell wall polysaccharide that occurs endogenously at distinct sites in a variety of tissues. Callose is also formed in response to stress involving cell membrane perturbation. In sections of chemically-fixed nodule tissue of the actinorhizal host, Datisca glomerata, callose was cytochemically detected within the Frankia-infected cortical cells, as an extensive network of wall material surrounding the microsymbiont, but not in uninfected cortical cells. Callose formation was completely inhibited within the infected cells when 2-deoxy-D-glucose, an inhibitor of callose formation, was included in the tissue fixative. The study concludes that callose deposition in the Datisca nodule infected zone is apparently a stress response to tissue preparation and fixation. However, the rapidity and extent of callose deposition primarily at the symbiotic interface in Frankia-infected cells suggests an unusual predisposition to biosynthesis of β-1,3-glucan in the nodule cortical cells that is related to their interaction with the microsymbiont.

Key words

Actinorhizal - β-1,3-glucan - callose - 2-deoxy-D-glucose - Datisca glomerata - Frankia - root nodule - symbiosis

1 Dedicated to Professor Walter Eschrich for his 75th birthday

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1 Dedicated to Professor Walter Eschrich for his 75th birthday

A. M. Berry

Department of Environmental Horticulture
University of California

One Shields Ave.
Davis, CA 95616
USA

Email: amberry@ucdavis.edu

Section Editor: A. Läuchli