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Plant Biol (Stuttg) 2002; 4(1): 70-76
DOI: 10.1055/s-2002-20438
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Three-Dimensional Reconstruction of the Stomatal Complex in Pinus canariensis Needles Using Serial Sections

G. Zellnig 1 , J. Peters 2 , M. S. Jiménez 2 , D. Morales 2 , D. Grill 1 , A. Perktold 1
  • 1 Institut für Pflanzenphysiologie, Universität Graz, Graz, Austria
  • 2 Dpto. Biología Vegetal, Universidad de La Laguna, La Laguna, Tenerife, Spain
Further Information

Publication History

June 8, 2001

November 13, 2001

Publication Date:
28 February 2002 (online)

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Abstract

The three-dimensional (3D) morphology of the stomata in leaves of Pinus canariensis is described with respect to the spatial arrangement of guard cells, subsidiary cells, polar and lateral cells. Serial semi-thin sections of the stomatal apparatus were digitally reconstructed and analysed with regard to the position, shape and size of the cell types involved. The stomatal complex consists of 16 cells with uniquely shaped polar and lateral cells. The polar cells form a kind of roof above the epistomatal chamber thereby reducing the surface aperture. The structural features of the stomatal complex differ from other Pinus species and are presumed to be an adaptation to extreme environmental conditions.

Abbreviations

3D: three-dimensional

2D: two-dimensional

Key words

Three-dimensional reconstruction - semi-thin section - stomatal complex - leaves - Pinus canariensis

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G. Zellnig

Institute of Plant Physiology

Schubertstr. 51
8010 Graz
Austria

Email: guenther.zellnig@uni-graz.at

Section Editor: H. Rennenberg