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Plant Biol (Stuttg) 2002; 4(3): 321-328
DOI: 10.1055/s-2002-32336
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Electron Transport Activities of Isolated Thylakoids from Wheat Plants Grown in Salicylic Acid

G. K. Sahu 1 , M. Kar 2 , S. C. Sabat 1
  • 1 Stress Biology Laboratory, Institute of Life Sciences, Bhubaneswar-751023, India
  • 2 Department of Botany, Utkal University, Bhubaneswar-751004, India
Further Information

Publication History

September 7, 2001

March 21, 2002

Publication Date:
20 June 2002 (online)

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Abstract

Wheat (Triticum aestivum cv. Sonalika) plants were grown with three different concentrations of salicylic acid (SA; 50/500/1000 μM) for 7 days and the effects on the level of thylakoid photochemical activities were examined. SA treatment stimulated photosystem II-catalyzed electron flow in all concentrations tested. Photosystem I-associated electron transport activity was stimulated at low concentrations of SA (50 μM) but at higher concentrations (500 and 1000 μM) the electron transport activity was drastically attenuated. Thylakoids isolated from the leaves of seedlings grown with high concentrations of SA (500 and 1000 μM) showed a substantial reduction in uncoupler (NH4Cl)-mediated stimulation in electron flow. In addition, they failed to support ADP-dependent stimulation of electron transport activity and induced a significant reduction in ATPase activity. Incubation of isolated thylakoids with SA, however, had no effect on thylakoid photofunction, indicating no direct effect of SA on photoelectron transport activity. Furthermore, high concentrations of SA specifically reduce the thylakoid cytochrome f554 level. The results suggest that SA, depending on its concentration, imparts differential effects on the photofunction of thylakoids. A low concentration of SA favours photosynthetic activity while the high concentration induces drastic attenuation of photosynthetic activity because of the decline in cytochrome f554.

Abbreviations

DCBQ: 2,6-dichloro-p-benzoquinone

DCMU: 3-(3′,4′ dichlorophenyl)-1′,1′-dimethylurea

DCIPH2: 2,6-dichlorophenolindophenol-reduced

FeCN: ferricyanide

Hepes: (N-[2-Hydroxyethyl] piperazine-N′-[2-ethanesulfonic acid])

TMPDH2: N,N,N,′N′-tetramethyl-p-phenylenediamine

Key words

ATPase - electron transport - photophosphorylation - salicylic acid - thylakoids - wheat

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S. C. Sabat

Stress Biology Laboratory
Institute of Life Sciences
Nalco Square

South Eastern Railway Project Post Office
Chandrasekharpur
Bhubaneswar-751023
Orissa
India

Email: scsabat@yahoo.com

Section Editor: U. Lüttge