Diayangambin Exerts Immunosuppressive and Anti-Inflammatory Effects in vitro and in vivo

Abstract

In this study, the furofuran lignan (+)-diayangambin [tetrahydro-1,4-bis(3,4,5-trimethoxyphenyl)-(1R)-1α,3aβ,4α,6aβ-1H,3H-furo [3,4-c]furan] was evaluated in vitro and in vivo for its immunomodulatory and anti-inflammatory efficacy. Human mononuclear cell proliferation was inhibited by diayangambin with an IC₅₀ value of 1.5 (0.5 - 2.8) μM. In addition, the compound reduced for 40.8 % prostaglandin E₂ generation in stimulated RAW 264.7 macrophage cell line at 10 μM. In vivo, a clear reduction of ear swelling was observed when diayangambin (40 mg/kg) was administered orally to 2,4-dinitrofluorobenzene-treated mice. The inhibition of swelling was associated with a reduction of leukocyte infiltration determined as myeloperoxidase activity. In the carrageenan mouse paw edema model, diayangambin significantly suppressed inflamed paw volume and prostaglandin E₂ levels. Our findings indicate the potential interest of diayangambin in the treatment of immune and inflammatory responses.

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