Pulsatilloside A and Anemoside A3 Protect PC12 Cells from Apoptosis Induced by Sodium Cyanide and Glucose Deprivation

Xiang-Dong Gao; Wen-Cai Ye; Albert C. H. Yu; Yun Zhang; Ren-Xiang Tan; Ming Li; W. L. Wendy Hsiao

doi:10.1055/s-2003-37705

Planta Medica, Table of Contents

Planta Med 2003; 69(2): 171-174
DOI: 10.1055/s-2003-37705

Letter

Pulsatilloside A and Anemoside A₃ Protect PC12 Cells from Apoptosis Induced by Sodium Cyanide and Glucose Deprivation

Xiang-Dong Gao¹ ^{, 2} , Wen-Cai Ye³ , Albert C. H. Yu⁴ , Yun Zhang³ , Ren-Xiang Tan² , Ming Li¹ , W. L. Wendy Hsiao⁴

¹School of Biopharmaceutical, China Pharmaceutical University, Nanjing, China
²Institute of Functional Biomolecule, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, China
³Department of Phytochemistry, China Pharmaceutical University, Nanjing, China
⁴Department of Biology, The Hong Kong University of Science and Technology, Hong Kong, China

Abstract

Using sodium cyanide (NaCN) and glucose deprivation induced cell injury in PC12 as an injury model, we investigated the protective effects of pulsatilloside A and anemoside A₃ on neurons. The results showed that PC12 cells under the NaCN-injury and glucose deprivation would undergo apoptosis. Additions of pulsatilloside A and anemoside A₃, at dosages ranging from 0.1, 1 and 10 μg/ml, protected PC12 cells from apoptosis determined by MTT, LDH release analysis, and flow cytometry measurement.

Abbreviations

β-AP:β-amyloid protein

FASC:flow cytometry

LDH:lactate dehydrogenase

NGF:nerve growth factor

TEM:transmission electron microscope

Full Text

References

1 Behl C. Apoptosis and Alzheimer's disease. J Neural Transm. 2000; 107 1325-44
2 Jiangsu New Medical College. Dictionary of Traditional Chinese Medicine. Shanghai Science and Technology Publication Ltd. Shanghai; 1997: pp. 704-5
3 Ye W C, Ji N N, Zhao S X, Liu J H, Ye T, McKervey M A, Stevenson P. Triterpenoids from Pulsatilla chinensis . Phytochemistry 1996 42: 799-802
4 Chen W K, Liu Q, Chen L, Kasai R, Tanaka Q. Saponins from Pulsatilla chinensis . Acta Chimica Sinica. 1990; 48 501
5 Spear N, Estevez A G, Barbeito L, Beckman J S, Johnson G V. Nerve growth factor protects PC12 cells against peroxynitrite-induced apoptosis via a mechanism dependent on phosphatidylinositol 3-kinase. Journal of Neurochemistry. 1997; 69 53-9
6 Liu J Q, Zhang X G. Study on the anti-apoptotic mechanism of Ginsenoside Rg1 in cultured cortical neurons. Acta Pharmaceutica Sinica. 1997; 32 406-10
7 Yuan J Y, Yankner B A. Apoptosis in the nervous system. Nature. 2000; 407 802-9
8 Shimohama S. Apoptosis in Alzheimer's disease - an update. Apoptosis. 2000; 5 9-16
9 Mills E M, Gunasekar P G, Pavlakovic G, Isom G E. Cyanide-induced apoptosis and oxidative stress in differentiated PC12 cell. J Neurochem.. 1996; 67 1039-46
10 Kim S R, Sung S H, Kwon S W, Park J H, Huh H, Kim Y C. Dammarane derivatives protect cultured rat cortical cells from glutamate-induced neurotoxicity. J. of Pharmacy & Pharmacology. 2000; 52 1505-11
11 Li M, Gao X D, Wu W T. Protective Effect of Ginkgolides on Apoptosis in PC12 Cells Induceced by Cyanide and Glucose Deprivation. Pharmaceutical Biotechnology. 2000; 7 46-9
12 Mosmann T. Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. J. Immunol Methods. 1983; 65 55-63
13 Maunsbach A B. 9. Electron Microscopy. In: Celis JE, editor Academic Press Vol. 3, 1998: 249-267
14 Hotz M A, Gong J, Traganos F, Darznkiewicz Z. Folw Cytometric Detection of in Situ DNA Degradation and Chromation Changes. Cytometry. 1994; 15 237-44

W. L. Wendy Hsiao

Department of Biology

The Hong Kong University of Science and Technology

Kowloon, Hong Kong

Email: bowhsiao@ust.hk

Fax: 852-23581559