Planta Med 2003; 69(2): 171-174
DOI: 10.1055/s-2003-37705
Letter
© Georg Thieme Verlag Stuttgart · New York

Pulsatilloside A and Anemoside A3 Protect PC12 Cells from Apoptosis Induced by Sodium Cyanide and Glucose Deprivation

Xiang-Dong Gao1 , 2 , Wen-Cai Ye3 , Albert  C. H. Yu4 , Yun Zhang3 , Ren-Xiang Tan2 , Ming Li1 , W. L. Wendy Hsiao4
  • 1School of Biopharmaceutical, China Pharmaceutical University, Nanjing, China
  • 2Institute of Functional Biomolecule, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, China
  • 3Department of Phytochemistry, China Pharmaceutical University, Nanjing, China
  • 4Department of Biology, The Hong Kong University of Science and Technology, Hong Kong, China
Further Information

Publication History

Received: May 21, 2002

Accepted: September 22, 2002

Publication Date:
07 March 2003 (online)

Abstract

Using sodium cyanide (NaCN) and glucose deprivation induced cell injury in PC12 as an injury model, we investigated the protective effects of pulsatilloside A and anemoside A3 on neurons. The results showed that PC12 cells under the NaCN-injury and glucose deprivation would undergo apoptosis. Additions of pulsatilloside A and anemoside A3, at dosages ranging from 0.1, 1 and 10 μg/ml, protected PC12 cells from apoptosis determined by MTT, LDH release analysis, and flow cytometry measurement.

Abbreviations

β-AP:β-amyloid protein

FASC:flow cytometry

LDH:lactate dehydrogenase

NGF:nerve growth factor

TEM:transmission electron microscope

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W. L. Wendy Hsiao

Department of Biology

The Hong Kong University of Science and Technology

Kowloon, Hong Kong

Email: bowhsiao@ust.hk

Fax: 852-23581559