Autoantibodies against Oxidized LDL and Serum Total Antioxidant Status in Active Cyclists and Ex-Cyclists

Abstract

There is an apparent paradox between the benefits of aerobic exercise and the potentially deleterious effects of increased free radicals and decreased circulating antioxidants generated during exercise. To assess the oxidative/antioxidative status in competitive cyclists and ex-cyclists, we measured two markers not well studied in these situations, serum total antioxidant status (TAS) and antibodies against oxidized LDL (AuAb-ox-LDL) in 18 competitive male cyclists, 10 ex-competitive cyclists and 14 healthy males. AuAb-ox-LDL was evaluated by enzyme immunoassay, and serum TAS concentration was analyzed by spectrophotometry. Ex-cyclists had serum TAS levels statistically higher than the control group and cyclists group (p = 0.001 and p < 0.001, respectively). Active sportsmen showed significantly less AuAb-ox-LDL than healthy sedentary males, while there was a non-significant trend in the ex-cyclists to have lower AuAb-ox-LDL than the corresponding control group. AuAb-ox-LDL levels were not statistically different between the groups of active and previously active sporting men. There was a positive correlation between TAS and LDL-cholesterol in active cyclists under heavy training. In the ex-cyclist group, there was a negative correlation between serum TAS and the time elapsed since they had ended the competition. Competitive cycling decreases AuAb-ox-LDL levels, suggesting that it may decrease ox-LDL levels. After ending physical training, antioxidative status remains increased for at least one year, but the effect on AuAb-ox-LDL levels is lost.

Training Exercise - Oxidative Stress - Serum Total Antioxidant Status - Oxidized LDL - Autoantibodies Against ox-LDL - Cyclists - Ex-Cyclists

References

• 1 Morris J N, Kagan A, Pattison D C, Gardner M J. Incidence and prediction of ischaemic heart-disease in London busmen.  Lancet. 1966;  2 553-559
• 2 Roserman R H, Brand R J, Jenkins D, Friedman M, Straus R, Wurm M. Coronary heart disease in the Western Collaborative Group.  JAMA. 1975;  233 872-877
• 3 Cassel J, Heyden S, Bartel A G. et al . Occupation and physical activity and coronary heart disease.  Arch Intern Med. 1971;  128 920-928
• 4 Durstine J L, Haskell W L. Effects of exercise on plasma lipids and lipoproteins.  Exerc Sport Sci Rev. 1994;  22 477-521
• 5 Liu M, Bergholm R, Mäkimattila S, Lahdenpera S, Valkonen M, Hilden H, Yki-Järvinen H, Taskinen M. A marathon run increases the susceptibility of LDL to oxidation in vitro and modifies plasma antioxidants.  Am J Physiol. 1999;  276 E1083-E1091
• 6 Kishimoto H, Taniguchi A, Fukushima M, Sakai M, Tokuyama K, Oguma T, Nin K, Nagayta I, Hayashi R, Kawano M, Hayashi K, Tsukamoto Y, Okumura T, Nagasaka S, Mizutani H, Nakai Y. Effect of short-term low-intensity exercise on insulin sensitivity, insulin secretion, and glucose and lipid metabolism in non-obese Japanese type 2 diabetic patients.  Horm Metab Res. 2002;  34 27-31
• 7 Sjödin B, Westing Y H, Apple F S. Biochemical mechanisms for oxygen free radical formation during exercise.  Sports Med. 1990;  10 236-254
• 8 Bergholm R, Makimattila S, Valkonen M, Liu M L, Lahdenpera S, Taskinen M R, Sovijarvi A, Malmberg P, Yki-Jarvinen H. Intense physical training decreases circulating antioxidants and endothelium-dependent vasodilatation in vivo.  Atherosclerosis. 1999;  145 341-349
• 9 Leaf D A, Kleinman M T, Hamilton M, Deeitrick R W. The exercise-induced oxidative stress paradox: The effects of physical exercise training.  Am J Med Sci. 1999;  317 295-300
• 10 Gutteridge J M. Lipid peroxidation and antioxidants as biomarkers of tissue damage.  Clin Chem. 1995;  41 1819-1828
• 11 Sen C K. Oxidants and antioxidants in exercise.  J Appl Physiol. 1995;  79 675-686
• 12 Konig D, Wagner K H, Elmadfa I, Berg A. Exercise and oxidative stress: significance of antioxidants with reference to inflammatory, muscular, and systemic stress.  Exerc Immunol Rev. 2001;  7 108-133
• 13 Wang X L, Rainwater D L, VendeBerg J F, Mitchell B D, Mahaney M C. Genetic contributions to plasma total antioxidant activity.  Arterioscl Thromb Vasc Biol. 2001;  21 1190-1195
• 14 Ghiselli A, Serafini M, Natella F, Scaccini C. Total antioxidant capacity as a tool to assess redox status: critical view and experimental data.  Free Radical Biol Med. 2000;  29 1106-1114
• 15 Therond P, Bonnefont-Rousselot D, Davit-Spraul A, Conti M, Legrand A. Biomarkers of oxidative stress: an analytical approach.  Curr Opin Clin Nutr Metab Care. 2000;  3 373-384
• 16 Ross R. Atherosclerosis, an inflammatory disease.  N Engl J Med. 1999;  340 115-126
• 17 Orem C, Orem A, Uydu H A, Celik S, Erdol C, Kural B V. The effects of lipid-lowering therapy on low-density lipoprotein auto-antibodies: relationship with low-density lipoprotein oxidation and plasma total antioxidant status.  Coron Artery Dis. 2002;  13 65-71
• 18 Turk Z, Sesto M, Skodlar J, Ferencak G, Turk N, Stavljenic-Rukavina A. Soluble LDL-immune complexes in type 2 diabetes and vascular disease.  Horm Metabol Res. 2002;  34 196-201
• 19 Salonen J T, Yla-Herttuala S, Yamamoto R, Butler S, Korpela H, Salonen R, Nyyssonen K, Palinski W, Witztum J L. Autoantibody against oxidised LDL and progression of carotid atherosclerosis.  Lancet. 1992;  339 883-887
• 20 Inoue T, Uchida T, Kamishirado H, Takayanagi K, Hayashi T, Morooka S. Clinical significance of antibody against oxidized low density lipoprotein in patients with atherosclerotic coronary artery disease.  J Am Coll Cardiol. 2001;  37 775-779
• 21 Sanchez-Quesada J L, Ortega H, Payes-Romero A, Serrat-Serrat J, González-Sastre F, Lasunción M A, Ordonez-Llanos J. LDL from aerobically-trained subjects shows higher resistance to oxidative modification than LDL from sedentary subjects.  Atherosclerosis. 1997;  132 207-213
• 22 Williams P T, Krauss R M, Wood P D, Lindgren F T, Giotas C, Vranizan K M. Lipoprotein subfractions of runners and sedentary men.  Metabolism. 1986;  35 45-52
• 23 Johnson A, Collins P, Higgins I, Harrington D, Connolly J, Dolphin C, McCreery M, Brady L, O'Brien M. Psychological, nutritional and physical status of olympic road cyclists.  Br J Sports Med. 1985;  19 11-14
• 24 Giada F, Baldo-Enzi G, Balocchi M R, Zuliani G, Baroni L, Fellin R. Heparin-released plasma lipase activities, lipoprotein and apoprotein levels in young adult cyclists and sedentary men.  Int J Sports Med. 1988;  9 270-274
• 25 Ji L L. Exercise and oxidative stress: role of the cellular antioxidant systems.  Exerc Sport Sci Rev. 1995;  23 135-166
• 26 Sánchez-Quesada J L, Homs-Serradesanferm R, Serrat-Serrat J, Serra Grima J R, González Sastre F, Ordópez Llanos J. Increase of LDL susceptibility to oxidation occurring after intense long duration aerobic exercise.  Atherosclerosis. 1995;  118 297-305
• 27 Kujala U M, Ahotupa M, Vasankari T, Kaprio J, Tikkanen M J. Low LDL oxidation in veteran endurance athletes.  Scand J Med Sci Sports. 1996;  6 303-308
• 28 Vasankari T J, Kujala U M, Vasankari T M, Ahotupa M. Reduced oxidized LDL levels after a 10-month exercise program.  Med Sci Sports Exerc. 1998;  30 1496-1501
• 29 Pincemail J, Lecomte J, Castiau J P, Collard E, Vasankari T, Cherami-Bien J, Limet R, Defraigue J. Evaluation of autoantibodies against oxidized LDL and antioxidant status in top soccer and basketball players after 4 months of competition.  Free Radic Biol Med. 2000;  28 559-565
• 30 Vasankari T J, Kujala U M, Vasankari T M, Vuorimaa T, Ahotupa M. Effects of acute prolonged exercise on serum and LDL oxidation and antioxidant defences.  Free Radic Biol Med. 1997;  22 509-513
• 31 Brites F D, Evelson P A, Christiansen M G, Nicol M F, Basilico M J, Wikinski R W, Llesuy S F. Soccer players under regular training show oxidative stress but an improved plasma antioxidant status.  Clin Sci (Lond). 1999;  96 381-385
• 32 Subudhi A W, Davis S L, Kipp R W, Askew W. Antioxidant status and oxidative stress in elite alpine ski racers.  Intern J of Sport Nutr and Exerc Metab. 2001;  11 32-41