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DOI: 10.1055/s-2003-45146
© Georg Thieme Verlag Stuttgart · New York
Transgenic Ginseng Cell Lines That Produce High levels of a Human Lactoferrin
Publikationsverlauf
Received: March 26, 2003
Accepted: August 16, 2003
Publikationsdatum:
09. Januar 2004 (online)
![](https://www.thieme-connect.de/media/plantamedica/200311/lookinside/thumbnails/10.1055-s-2003-45146-1.jpg)
Abstract
In order to produce a human lactoferrin (hLf) protein in cultured plant cells, we developed Korean ginseng (Panax ginseng) cell line using an oxidative stress-inducible peroxidase (SWPA2) promoter and characterized the production of human lactoferrin in cultured cells. A construct containing a targeting signal peptide from tobacco endoplasmic reticulum fused to human lactoferrin cDNA under the control of SWPA2 promoter was engineered. Transgenic Korean ginseng cell lines that produced a recombinant hLf protein were successfully generated and confirmed by PCR and Southern blot analyses. Western blot and ELISA analyses showed that hLf protein was synthesized in the transgenic cells. The production of hLf showed a maximal level (up to 3.0 % of total soluble protein) in the stationary phase of callus cultures. These results suggest that the transgenic cell lines in this study will be biotechnologically useful for the commercial production of hLf protein in cell cultures, with no need for purification.
Abbreviations
ELISA:enzyme-linked immunosorbent assay
TSP:total soluble protein
MS:Murashige and Skoog
DAS:days after subculture
Key words
Panax ginseng - Araliaceae - high expression promoter - human lactoferrin - recombinant protein - transgenic cell cultures
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Haeng-Soon Lee, PhD
Laboratory of Plant Cell Biotechnology
Korea Research Institute of Bioscience and Biotechnology (KRIBB)
Oun-Dong 52
Yusong-gu
Daejeon 305-806
Korea
Fax: +82-42-860-4608
eMail: hslee@kribb.re.kr