Planta Med 2003; 69(11): 1005-1008
DOI: 10.1055/s-2003-45146
Original Paper
Biochemistry and Molecular Biology
© Georg Thieme Verlag Stuttgart · New York

Transgenic Ginseng Cell Lines That Produce High levels of a Human Lactoferrin

Suk-Yoon Kwon2 , Seung-Hyun Jo1 , Ok-Sun Lee1 , Sun-Mee Choi1 , Sang-Soo Kwak2 , Haeng-Soon Lee1
  • 1Laboratory of Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
  • 2Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea
Further Information

Publication History

Received: March 26, 2003

Accepted: August 16, 2003

Publication Date:
09 January 2004 (online)

Abstract

In order to produce a human lactoferrin (hLf) protein in cultured plant cells, we developed Korean ginseng (Panax ginseng) cell line using an oxidative stress-inducible peroxidase (SWPA2) promoter and characterized the production of human lactoferrin in cultured cells. A construct containing a targeting signal peptide from tobacco endoplasmic reticulum fused to human lactoferrin cDNA under the control of SWPA2 promoter was engineered. Transgenic Korean ginseng cell lines that produced a recombinant hLf protein were successfully generated and confirmed by PCR and Southern blot analyses. Western blot and ELISA analyses showed that hLf protein was synthesized in the transgenic cells. The production of hLf showed a maximal level (up to 3.0 % of total soluble protein) in the stationary phase of callus cultures. These results suggest that the transgenic cell lines in this study will be biotechnologically useful for the commercial production of hLf protein in cell cultures, with no need for purification.

Abbreviations

ELISA:enzyme-linked immunosorbent assay

TSP:total soluble protein

MS:Murashige and Skoog

DAS:days after subculture

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Haeng-Soon Lee, PhD

Laboratory of Plant Cell Biotechnology

Korea Research Institute of Bioscience and Biotechnology (KRIBB)

Oun-Dong 52

Yusong-gu

Daejeon 305-806

Korea

Fax: +82-42-860-4608

Email: hslee@kribb.re.kr