Planta Med 2003; 69(11): 1057-1059
DOI: 10.1055/s-2003-45157
Letter
© Georg Thieme Verlag Stuttgart · New York

Tanshinone IIA from Salvia miltiorrhiza Inhibits Inducible Nitric Oxide Synthase Expression and Production of TNF-α, IL-1β  and IL-6 in Activated RAW 264.7 Cells

Seon-Il Jang1 , Seung-Il Jeong1 , Kang-Ju Kim1 , Hyung-Jin Kim1 , Hyeon-Hee Yu2 , Raekil Park3 , Hyung-Min Kim4 , Yong-Ouk You2
  • 1Department of Oral Microbiology, School of Dentistry, Wonkwang University, Iksan, South Korea
  • 2Department of Oral Biochemistry and VCRC, School of Dentistry, Wonkwang University, Iksan, South Korea
  • 3Department of Microbiology and VCRC, School of Medicine, Wonkwang University, Iksan, South Korea
  • 4Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, Seoul, South Korea
This paper was supported by VestibuloCoclear Research Center and a grant No. R01-2001-000-00133-0 from the Basic Research Program of the KOSEF, and also supported by Wonkwang University, 2003
Weitere Informationen

Publikationsverlauf

Received: March 20, 2003

Accepted: August 16, 2003

Publikationsdatum:
09. Januar 2004 (online)

Abstract

The inhibitory effects of tanshinone IIA, a diterpene isolated from Salvia miltiorrhiza root, on the production of nitric oxide (NO), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), and the expression of inducible nitric oxide synthase (iNOS) were investigated in activated RAW 264.7 cells. This compound markedly inhibited the production of NO, IL-1β and TNF-α, and suppressed the expression of iNOS in a dose-dependent manner. These results suggest that the traditional use of S. miltiorrhiza as an anti-inflammatory herbal medicine may be explained, in part, by the inhibition of NO, IL-1β, IL-6 and TNF-α production, and expression of iNOS.

References

  • 1 Kim N Y, Kang T H, Pae H O, Choi B M, Chung H T, Myung S W, Song Y S, Sohn D H, Kim Y C. In vitro inducible nitric oxide synthesis inhibitors from Alismatis Rhizoma.  Biol Pharm Bull. 1999;  22 1147-9
  • 2 Watanabe K, Jinnouchi K, Hess A, Michel O, Yagi T. Detection of apoptotic change in the lipopolysaccharide (LPS)-treated cochlea of guinea pigs.  Hearing Res. 2001;  158 116-22
  • 3 Hirose M, Ishihara K, Saito A, Nakagawa T, Yamada S, Okuda K. Expression of cytokines and inducible nitric oxide synthase in inflamed gingival tissue.  J Periodontol. 2001;  72 590-7
  • 4 Song Y S, Park E H, Hur G M, Ryu Y S, Lee Y S, Lee J Y, Kim Y M, Jin C. Caffeic acid phenethyl ester inhibits nitric oxide synthase gene expression and enzyme activity.  Cancer Lett. 2002;  175 53-61
  • 5 Kim J K. Illustrated natural drugs encyclopedia. Seoul; Namsandan Publishers 1989: p. 160
  • 6 Ryu S Y, No Z, Kim S H, Ahn J W. Two novel abietane diterpenes from Salvia miltiorrhiza .  Planta Med. 1997;  63 44-6
  • 7 Ouyang X, Takahashi K, Komatsu K, Nakamura N, Hattori M, Baba A, Azuma J. Protective effect of Salvia miltiorrhiza on angiotensin II-induced hypertrophic responses in neonatal rat cardiac cells.  Jpn J Pharmacol. 2001;  87 289-96
  • 8 Heitmeier M R, Scarim A L, Corbett J A. Double-stranded RNA-induced inducible nitric-oxide synthase expression and interleukin-1 release by murine macrophages requires NF-κB activation.  J Biol Chem. 1998;  273 15 301-7
  • 9 So H S, Park R K, Oh H M, Pae H O, Lee J H, Chai K Y, Chung S Y, Chung H T. The methanol extract of Spiraea prunifolia var. simpliciflora root inhibits the generation of nitric oxide and superoxide in RAW 264.7 cells.  J Ethnopharmacol. 1999;  68 209-17

Prof. Dr. Yong-Ouk You

Department of Oral Biochemistry

School of Dentistry

Wonkwang University

344-2 Shinyong-dong

Iksan

Chonbuk 570-749

South Korea

Telefon: +82-63-850-6926

Fax: +82-63-850-7157

eMail: hope7788@wonkwang.ac.kr