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Horm Metab Res 2004; 36(1): 34-38
DOI: 10.1055/s-2004-814100
Original Clinical
© Georg Thieme Verlag Stuttgart · New York

Effects of Imidapril, an Angiotensin-converting Enzyme Inhibitor, on Insulin Sensitivity and Responsiveness in Streptozotocin-induced Diabetic Rats

A.  Y.  I.  Viana1 , Y.  Oshida1, 2 , Y-Q.  Han2 , K.  Koshinaka1 , Y.  Sato1, 2
  • 1Department of Sports Medicine, Graduate School of Medicine, Nagoya University, Nagoya, Japan
  • 2Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya, Japan
Weitere Informationen

Publikationsverlauf

Received 17 September 2002

Accepted after second revision 18 September 2003

Publikationsdatum:
25. Februar 2004 (online)

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Abstract

We have studied the effect of imidapril, an angiotensin-converting enzyme inhibitor, on streptozotocin-induced diabetic rats. A sequential euglycemic hyperinsulinemic clamp procedure was used (insulin infusion rates: 3 and 30 mU/kg BW/min) in 30 diabetic rats. The rats were divided in 6 groups: a control group, a control group with N-monomethyl-L-arginine (L-NMMA, 1 mg/kg/min, a nitric oxide synthase inhibitor) infusion, a streptozotocin-induced diabetic group, a diabetic group with L-NMMA infusion, a diabetic group involving imidapril infusion (5 μg/kg/min), and a diabetic group involving simultaneous imidapril and L-NMMA infusion. Glucose concentrations were maintained around 140 mg/dl during the clamp studies. Plasma insulin levels during the 3 and 30 mU/kg BW/min insulin infusions were 30 and 400 μU/ml, respectively. Glucose infusion rates (GIR) in STZ-induced diabetic rats showed a significant decrease compared to controls. At both insulin infusion rates, imidapril-infused diabetic rats showed an increased GIR, compared with the saline infused ones. There was no significant difference in GIR between L-NMMA and saline infusion in diabetic rats. Simultaneous infusion of imidapril and L-NMMA did not significantly decrease GIR with low-dose insulin infusion, but the increase in GIR induced by imidapril with high-dose insulin infusion was impaired by 100 % by L-NMMA infusion in diabetic rats. These results suggest that imidapril may improve insulin action, in part, via nitric oxide.

Key words

Angiotensin-Converting Enzyme Inhibitor - Euglycemic Clamp Technique - L-NMMA - Streptozotocin

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Y. Sato, M. D, Ph. D.

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