In Vitro Antimalarial Activity of Biflavonoids from Wikstroemia indica

Shinyu Nunome; Aki Ishiyama; Miyuki Kobayashi; Kazuhiko Otoguro; Hiroaki Kiyohara; Haruki Yamada; Satoshi Omura

doi:10.1055/s-2004-815462

Planta Med 2004; 70(1): 76-78
DOI: 10.1055/s-2004-815462

Letter

In Vitro Antimalarial Activity of Biflavonoids from Wikstroemia indica

Shinyu Nunome¹ , Aki Ishiyama² , Miyuki Kobayashi² , Kazuhiko Otoguro² , Hiroaki Kiyohara¹ , Haruki Yamada¹ ^, ² , Satoshi Omura¹ ^, ²

¹The Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan
²Research Center for Tropical Diseases, The Kitasato Institute, Tokyo, Japan

This work was supported, in part, by funds from the UNDP/World Bank/WHO special Program for Research and Training in Tropical Disease (grant ID 990 806 and IDA10124), and Grants-in Aid for Scientific Research (A) from the Ministry of Education, Culture, Sports, Science and Technology, Japan. A part of work was supported by the 21st Century COE Program, Ministry of Education, Culture, Sports, Science and Technology (MEXT)

Abstract

In our investigation of in vitro antimalarial screening of medicinal herbal extracts, the n-BuOH extract from the root of Wikstroemia indica showed a potent inhibitory effect. Fractionation of the active extract led to the isolation of two biflavonoids, sikokianin B (1) and sikokianin C (2) with IC₅₀ values 0.54 μg/mL and 0.56 μg/mL, respectively, against the chloroquine-resistant strain of Plasmodium falciparum. This is the first report of the biological activity of 1 and 2. As the structure of l has remained unsettled, we confirmed the conformation by ¹H- and ¹³C-NMR.

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References

1 Niwa M, Jiang F P, Hirata Y. Two new C-3/C-3′′-biflavanones from Wikstroemia sikokiana . Chem Pharm Bull. 1986; 34 3631-4
2 Baba K, Taniguchi M, Kozawa M. Three biflavonoids from Wikstroemia sikokiana . Phytochemistry. 1994; 37 879-83
3 State Pharmaceutical A dministration. Zhonghua Bencao. Shanghai Science and Technology Press China; 1998
4 Otoguro K, Kohana A, Manabe C, Ishiyama A, Ui H, Shiomi K, Yamada H, Omura S. Potent antimalarial activities of polyether antibiotic, X-206. J Antibiotics. 2001; 54 658-63

Prof. Dr. H. Yamada

The Kitasato Institute for Life Sciences

Kitasato University

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Email: yamada@lisci.kitasato-u.ac.jp