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Plant Biol (Stuttg) 2004; 6(3): 358-368
DOI: 10.1055/s-2004-817893
Original Paper

Georg Thieme Verlag Stuttgart KG · New York

Hg2+ Reacts with Different Components of the NADPH : Protochlorophyllide Oxidoreductase Macrodomains

K. Solymosi1 , K. Lenti1 , B. Myśliwa-Kurdziel2 , J. Fidy3 , 4 , K. Strzałka2 , B. Böddi1
  • 1Department of Plant Anatomy, Eötvös University, Budapest, Hungary
  • 2Department of Plant Physiology and Biochemistry, Jagiellonian University, Krakow, Poland
  • 3Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary
  • 4MTA-SE Research Group for Biophysics, Hungarian Academy of Science, Budapest, Hungary
Weitere Informationen

Publikationsverlauf

Publikationsdatum:
14. Mai 2004 (online)

   Lizenzen und Reprints

Abstract

The molecular background of Hg2+-induced inhibition of protochlorophyllide (Pchlide) photoreduction was investigated in homogenates of dark-grown wheat leaves. Our earlier work showed that 15 min incubation with 10-2 M Hg2+ completely inhibits the activity of NADPH : Pchlide oxidoreductase ([Lenti et al., 2002]). Detailed analysis of spectra recorded at 10 K indicated the appearance of emission bands at 638 and 650 nm, which are characteristic for NADP+-Pchlide complexes. Fluorescence emission spectra recorded with different excitation wavelengths, fluorescence lifetime measurements and the analysis of acetone extractions revealed that Hg2+ can also react directly with Pchlide, resulting in protopheophorbide formation. At 10-3 M Hg2+, the phototransformation was complete but the blue shift of the chlorophyllide emission band speeded up remarkably. This indicates oxidation of the NADPH molecules that have a structural role in keeping together the etioplast inner membrane components. We suggest a complex model for the Hg2+ effect: depending on concentration it can react with any components of the NADPH : Pchlide oxidoreductase macrodomains.

Key words

Mercury - Hg2+ - protochlorophyllide - NADPH - protopheophorbide - Shibata shift - activity loss

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B. Böddi

Department of Plant Anatomy
Eötvös University

Pázmány P. sétány 1/C

Budapest H-1117

Hungary

eMail: bbfotos@ludens.elte.hu

Guest Editor: F. Loreto