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DOI: 10.1055/s-2004-820990
© Georg Thieme Verlag KG Stuttgart · New York
Hyperthermia Increases Exercise-induced Oxidative Stress
Publikationsverlauf
Accepted after revision: March 2, 2004
Publikationsdatum:
26. August 2004 (online)
![](https://www.thieme-connect.de/media/sportsmed/200503/lookinside/thumbnails/10.1055-s-2004-820990-1.jpg)
Abstract
The purpose of this investigation was to examine oxidative markers after exercise in a hyperthermic environment (35 °C, 70 % RH) (Hot) versus a neutral environment (25 °C, 40 % RH) (Con). Hyperthermia may exacerbate oxidative stress by uncoupling the mitochondrial respiratory chain or by inhibiting antioxidant defense mechanisms, but this has not been assessed in vivo. Six male subjects performed low-intensity exercise (50 % VO2max) on a treadmill in Hot until a core temperature of 39.5 °C was reached, and for an equivalent time in Con. Blood samples were drawn before and immediately after exercise and at 8 min and 15 min following exercise. Samples were analyzed for F2 isoprostanes (FIP), lipid hydroperoxides (LPO), and lactate. A 2 × 4 repeated measures ANOVA was used to test for treatment, time, and interaction effects for FIP, LPO, and lactate. Differences in VO2 were tested with Student's t-test. Significance was set at p < 0.05. Oxygen consumption was not significantly different between Hot and Con. The pattern of change of FIP and lactate in Hot was significant versus exercise in Con. LPO was significantly elevated over time in both Hot and Con, but the pattern of change was not significantly different. Ending core temperatures and heart rates were significantly elevated in Hot versus Con. These data indicate that hyperthermia increases oxidative stress and selectively affects specific lipid markers, independent of oxygen consumption.
Key words
Lipid peroxidation - F2 isoprostanes - lipid hydroperoxides - lactate
References
- 1 Brooks G A, Hittleman K J, Faulkner J A, Beyer R E. Tissue temperatures and whole-animal oxygen consumption after exercise. Am J Physiol. 1971; 221 427-431
- 2 Dill D B, Costill D L. Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol. 1974; 37 247-248
- 3 Febbraio M. Does muscle function and metabolism affect exercise performance in the heat?. Exerc Sports Sci Rev. 2000; 28 171-176
- 4 Flanagan S W, Moseley P, Buettner G R. Increased flux of free radicals in cells subjected to hyperthermia: detection by electron paramagnetic resonance spin trapping. FEBS Letters. 1998; 431 285-286
- 5 Freeman M L, Spitz D R, Meredith M J. Does heat shock enhance oxidative stress? Studies with ferrous and ferric iron. Rad Res. 1990; 124 288-293
- 6 Gleeson M. Temperature regulation during exercise. Int J Sports Med. 1998; 19 96-99
- 7 Gutmann I, Wahlefeld A W. Lactate Determination with Lactate Dehydrogenase and NAD. 2nd ed. New York; Academic 1974: 1464-1468
- 8 Hall D, Buettner G R, Matthes R D, Gisolfi C V. Hyperthermia stimulates nitric oxide formation: electron paramagnetic resonance detection of NO-heme in blood. J Appl Physiol. 1994; 77 548-553
- 9 Hargreaves M, Febbraio M. Limits to exercise performance in the heat. Int J Sports Med. 1998; 19 115-116
- 10 Hass M A, Massaro D. Regulation of the synthesis of superoxide dismutases in rat lungs during oxidant and hyperthermic stresses. J Biol Chem. 1988; 263 776-781
- 11 Hellsten Y, Svensson M, Sjodin B, Smith S, Christensen A, Richter E A, Bangsbo J. Allantoin formation and urate and glutathione exchange in human muscle during submaximal exercise. Free Rad Biol Med. 2001; 31 1313-1322
- 12 Krotkiewski M, Brzezinska Z, Liu B, Grimby G, Palm S. Prevention of muscle soreness by pretreatment with antioxidants. Scand J Med Sci Sports. 1994; 4 191-199
- 13 Lord-Fontaine S, Averill-Bates D A. Heat shock inactivates cellular antioxidant defences against hydrogen peroxide: protection by glucose. Free Rad Biol Med. 2002; 32 752-765
- 14 Mastaloudis A, Leonard S, Traber M G. Oxidative stress in athletes during extreme endurance exercise. Free Rad Biol Med. 2001; 31 911-922
- 15 McAnulty S R, McAnulty L, Nieman D C, Dumke C L, Morrow J D, Utter A C. et al . Consumption of blueberry polyphenols reduces exercise-induced oxidative stress compared to vitamin C. Nutr Res. 2004; 24 209-221
- 16 Mills P C, Smith N C, Casas I, Harris P, Harris R C, Marlin D J. Effects of exercise intensity and environmental stress on indices of oxidative stress and iron homeostasis during exercise in the horse. Eur J Appl Physiol. 1996; 74 60-66
- 17 Mitchell J B, Russo A. Thiols, thiol depletion, and thermo-sensitivity. Rad Res. 1983; 95 471-485
- 18 Mohazzab K M, Kaminski P, Wolin M. Lactate and PO2 modulate superoxide anion production in bovine cardiac myocytes. Circ. 1997; 96 614-620
- 19 Morrow J D, Roberts L J. The isoprostanes: unique bioactive products of lipid peroxidation. Prog Lipid Res. 1997; 36 1-21
- 20 Nieman D C, Henson D, McAnulty S R, McAnulty L, Swick N S, Utter A C. et al . Influence of vitamin C supplementation on oxidative and immune changes after an ultramarathon. J Appl Physiol. 2002; 92 1970-1977
- 21 Nourooz-Zadeh J, Tajaddini-Sarmadi J, Ling E, Wolff S. Low-density lipoprotein is the major carrier of lipid hydroperoxides in plasma. Bio J. 1996; 313 781-786
- 22 Parthasarathy S, Santanam N, Ramachandran S, Meilhac O. Potential role of oxidized lipids and lipoproteins in antioxidant defense. Free Rad Res. 2000; 33 197-215
- 23 Rehncrona S, Hauge H N, Siesjo B K. Enhancement of iron-catalyzed free radical formation by acidosis in brain homogenates: Difference in effect by lactic acid and CO2. J Cereb Blood Flow Metab. 1989; 9 65-70
- 24 Ryan A J, Gisolfi C V, Mosely P L. Synthesis of 70 K stress protein by human leukocytes: effect of exercise in the heat. J Appl Physiol. 1991; 70 466-471
- 25 Ryan M, Grayson L, Clarke D J. The total antioxidant capacity of human serum measured using enhanced chemiluminescence is almost completely accounted for by urate. Ann Clin Biochem. 1997; 34 688-689
- 26 Salo D C, Donovan C M, Davies K JA. HSP70 and other possible heat shock or oxidative stress proteins are induced in skeletal muscle, heart, and liver during exercise. Free Rad Biol Med. 1991; 11 239-246
- 27 Satoh K, Sakagami H, Nakamura K. Enhancement of radical intensity and cytotoxic activity of ascorbate by hyperthermia. Anticancer Res. 1996; 16 2987-2991
- 28 Siraki A G, O'Brien P J. Prooxidant activity of free radicals derived from phenol-containing neurotransmitters. Toxicol. 2002; 177 81-90
- 29 Sjodin B, Westing-Hellsten Y, Apple F. Biochemical mechanisms for oxygen free radical formation during exercise. Sports Med. 1990; 10 236-254
- 30 Viguie C, Frei B, Shigenaga M, Ames B N, Packer L, Brooks G A. Antioxidant status and indexes of oxidative stress during consecutive days of exercise. J Appl Physiol. 1993; 75 566-572
- 31 Vina J, Gomez-Cabrera M, Lloret A, Marquez R, Minana J B, Pallardo F V, Sastre J. Free radicals in exhaustive exercise: mechanisms of production, and protection by antioxidants. IUBMB Life. 2000; 50 271-277
- 32 Waring W S, Convery A, Mishra V, Shenkin A, Webb D J, Maxwell S R. Uric acid reduces exercise-induced oxidative stress in healthy adults. Clin Sci. 2003; 105 425-430
- 33 Wolff S. Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. Meth Enzymol. 1994; 233 182-189
S. R. McAnulty
Department of Health, Leisure, and Exercise Science · Appalachian State University
Boone, NC 28608
USA
Telefon: + 8282627151
Fax: + 82 82 62 31 38
eMail: mcanltysr@appstate.edu