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Int J Sports Med 2005; 26(9): 732-738
DOI: 10.1055/s-2005-837447
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

Effect of a Short-Term Graded Exhaustive Exercise on the Susceptibility of Serum Lipids to Oxidation

A. Dayan1 , A. Rotstein2 , I. Pinchuk1 , A. Vodovicz3 , Z. Lencovski3 , D. Lichtenberg1 , O. Inbar2
  • 1Department of Physiology and Pharmacology, Tel-Aviv University, Sackler School of Medicine, Tel-Aviv, Israel
  • 2Department Life Sciences, Zinman College, Wingate Institute, Israel
  • 3Medical Department, Israeli Police Forces, Jerusalem, Israel
Further Information

Publication History

Accepted after revision: October 31, 2004

Publication Date:
15 March 2005 (online)

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Abstract

The objective of our study was to evaluate the effect of short-term intensive exercise on the susceptibility of serum lipids to ex-vivo peroxidation. We assessed the association between aerobic capacity, serum composition, and serum lipid oxidizability as well as the association between aerobic capacity and the effect of short-term maximal exercise on the kinetics of ex-vivo copper-induced peroxidation of serum lipids. The study involved 30 healthy male volunteers (age 22 - 39 years, BMI 19.4 - 29.8). Following 12-hr fasting, blood was withdrawn for determination of blood lipids, LDL, HDL, and TG, and Vitamin E, and for oxidizability assay of the serum lipids. Subsequently, each volunteer underwent an incremental all-out cardiopulmonary exercise stress test (CPET), performed on a motor-driven treadmill (Quinton Q65, USA). The test protocol was a modified Balke protocol. The results of this test were expressed in terms of mass-dependent maximal oxygen uptake (V·O2max, ml · kg-1 · min-1) and of ventilatory anaerobic threshold (VAT, ml · kg-1 · min-1). Immediately after exercise, blood was re-drawn for the determination of serum Vitamin E and for ex-vivo oxidizibility assay, expressed in terms of maximal absorption of oxidation products (ODmax, absorbance units), maximal rate of their production (Vmax, OD min-1) and the time at which the rate was maximal (tmax, min). Maximal graded exercise had no significant effect on the susceptibility of serum lipids to peroxidation as measured by ODmax (p = 0.38 at 245 nm, and 0.27 at 268 nm),Vmax (p = 0.34 at 245 nm, and 0.49 at 268 nm) and tmax (p = 0.17 at 245 nm, and 0.07 at 268 nm). Also no effect was found on the concentration of serum Vitamin E (p = 0.39). Aerobic capacity was not associated either with the susceptibility of serum lipids to ex-vivo peroxidation or with serum Vitamin E concentration. The present findings indicate that a short graded maximal exercise, lasting 8 - 12 min, is not sufficient to increase the susceptibility of the serum lipids to peroxidation. Thus it may be assumed that the antioxidant capacity of most healthy subjects provides proper protection from a short exhaustive exercise challenge. Also, aerobic capacity in the range represented by our subjects does not seem to influence the susceptibility of serum lipids to peroxidation.

Key words

Lipid peroxidation - free radicals - exercise - aerobic fitness - Vitamin E

References

  • 1 Balke B, Ware R W. An experimental study of physical fitness of Air Force personnel.  US Armed Forces Med J. 1959;  10 675-688
    PubMedSearch in Google Scholar
  • 2 Bassuk S S, Manson J E. Physical activity and the prevention of cardiovascular disease.  Curr Atheroscler Rep. 2003;  5 299-307
    PubMedSearch in Google Scholar
  • 3 Chandan K S, Packer L, Hanninen O. Exercise and Oxygen Toxicity. Amsterdam; Elsevier Science B. V. 1994
    Search in Google Scholar
  • 4 Chandan K S. Oxidants and antioxidants in exercise.  J Appl Physiol. 1995;  79 675-686
    PubMedSearch in Google Scholar
  • 5 Child R B, Wilkinson D M, Fallowfield J L, Donnelly A E. Elevated serum antioxidant capacity and plasma malondialdehyde concentration in response to stimulated half-marathon run.  Med Sci Sports Exerc. 1998;  30 1603-1607
    PubMedSearch in Google Scholar
  • 6 Esterbauer H, Jurgens G. Mechanistic and genetic aspects of susceptibility of LDL to oxidation.  Curr Opin Lipidol. 1993;  4 114-124
    PubMedSearch in Google Scholar
  • 7 Esterbauer H, Dieber-Rotheneder M, Stregl G, Waeg G. Role of Vitamin E in preventing the oxidation of low-density lipoprotein.  Am J Clin Nutr. 1991;  53 314-321
    PubMedSearch in Google Scholar
  • 8 Ginsburg G S, Agil A, O'Toole M, Rimm E, Douglas P S, Rifai N. Effects of a single bout of ultraendurance exercise on lipid levels and susceptibility of lipids to peroxidation in triathletes.  JAMA. 1996;  276 221-225
    CrossrefPubMedSearch in Google Scholar
  • 9 Groussard C, Rannou-Bekono F, Machefer G, Chevanne M, Vincent S, Sergrent O, Cillard J, Gratase-Delamarche A. Changes in blood lipid peroxidation markers and antioxidants after a single sprint anaerobic exercise.  Eur J Appl Physiol. 2003;  89 14-20
    CrossrefPubMedSearch in Google Scholar
  • 10 Hoffman R M, Garewal H S. Antioxidants and the prevention of coronary heart disease.  Arch Intern Med. 1995;  155 241-246
    CrossrefPubMedSearch in Google Scholar
  • 11 Inbar O, Oren A, Scheinowitz M, Rotstein A, Dlin R, Casaburi R. Normal cardiopulmonary exercise in 20 to 70-yr-old men.  Med Sci Sports Exerc. 1994;  26 538-546
    PubMedSearch in Google Scholar
  • 12 Jackson A S, Pollock M L. Prediction accuracy of body density, lean body weight, and total body volume equations.  Med Sci Sports. 1977;  9 107-115
    PubMedSearch in Google Scholar
  • 13 Jenkins R R. Exercise, oxidative stress, and antioxidants: a review.  Int J Sport Nutr. 1993;  3 356-375
    PubMedSearch in Google Scholar
  • 14 Kanter M M. Free radicals, exercise, and antioxidant supplementation.  Int J Sport Nutr. 1994;  4 205-220
    PubMedSearch in Google Scholar
  • 15 Kritharides L, Stocker R. The use of antioxidant supplements in coronary heart disease.  Atherosclerosis. 2002;  164 211-219
    CrossrefPubMedSearch in Google Scholar
  • 16 Kujala U M, Ahoutupa M, Vasankari T, Kaprio J, Tikkanen M J. Low LDL oxidation in veteran endurance athletes.  Scand J Med Sci Sports. 1996;  6 303-308
    PubMedSearch in Google Scholar
  • 17 Leaf D A, Kleinman M T, Hamilton M, Barstow T J. The effect of exercise intensity on lipid peroxidation.  Med Sci Sports Exerc. 1997;  29 1036-1039
    PubMedSearch in Google Scholar
  • 18 Leaf D A, Kleinman M T, Hamilton M, Deitrick R W. The Exercise-induced stress paradox: The effects of physical exercise training.  Am J Med Sci. 1999;  317 295-300
    CrossrefPubMedSearch in Google Scholar
  • 19 Li L J. Exercise, oxidative stress, and antioxidants.  Am J Sports Med. 1996;  24 20-24
    PubMedSearch in Google Scholar
  • 20 Liu M L, Bergholm R, Makimattila S, Lahdenpera S, Valkonen M, Hilden H, Yki-Jarvinen H, Taskinen M R. A marathon run increases the susceptibility of LDL to oxidation in vitro and modifies plasma antioxidants.  Am J Physiol. 1999;  276 1083-1091
    PubMedSearch in Google Scholar
  • 21 Margaritis I, Tessier F, Richard M. Marconnet P. No evidence of oxidative stress after a triathlon race in highly trained competitors.  Int J Sports Med. 1997;  18 186-190
    Thieme ConnectPubMedSearch in Google Scholar
  • 22 Marzatico F, Pansarsa O, Bertorelli L, Somenzini Della Valle G. Blood free radical antioxidant enzymes and lipid peroxides following long-distance and lactacidemic performances in highly trained aerobic and sprint athletes.  J Sports Med Phys Fitness. 1997;  37 235-239
    PubMedSearch in Google Scholar
  • 23 Ohkuwa T, Sato Y, Naoi M. Glutathione and reactive oxygen generation in tissues of young and old exercised rats.  Acta Physiol Scand. 1997;  159 237-244
    CrossrefPubMedSearch in Google Scholar
  • 24 Ortenblad N, Madsen K, Djurhuus M S. Antioxidants status and lipid peroxidation after short-term maximal exercise in trained and untrained humans.  Am J Physiol. 1997;  272 1258-1263
    PubMedSearch in Google Scholar
  • 25 Parks E J, German J B, Davis P A, Frankel E N, Kappagoda C T, Rutledge J C, Hyson D A, Schneeman B O. Reduced oxidative susceptibility of LDL from patients participating in an intensive atherosclerosis treatment program.  Am J Clin Nutr. 1998;  68 778-785
    PubMedSearch in Google Scholar
  • 26 Reznick A Z. Oxidative Stress in Skeletal Muscle. Basel, Switzerland; Birkhauser Verlag 1998
    Search in Google Scholar
  • 27 Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s.  Nature. 1993;  362 801-809
    CrossrefPubMedSearch in Google Scholar
  • 28 Rush J WE, Sandiford S D. Plasma glutathione peroxidase in healthy young adults: influence of gender and physical activity.  Clin Biochem. 2003;  36 345-351
    PubMedSearch in Google Scholar
  • 29 Sacheck J M, Milbury P E, Cannon J G, Roubenoff R. Effect of vitamin E and eccentric exercise in selected biomarkers of oxidative stress in young and elderly men.  Free Rad Biol Med. 2003;  34 1575-1588
    CrossrefPubMedSearch in Google Scholar
  • 30 Sanchez-Quesada J L, Ortega H, Payes-Romero A, Serrat-Serrat J, Gonzalez-Sastre F, Lasuncion M A, Ordonez-Llanos J. LDL from aerobically trained subjects shows higher resistance to oxidative modification than LDL from sedentary subjects.  Atherosclerosis. 1997;  132 207-213
    CrossrefPubMedSearch in Google Scholar
  • 31 Sanchez-Quesada J L, Serradesanferm R H, Serrat-Serrat J, Serra-Grima J R, Gonzalez-Sastre F, Ordonez-Llanos J. Increase of LDL susceptibility to oxidation occurring after intense, long duration aerobic exercise.  Atherosclerosis. 1995;  118 297-305
    CrossrefPubMedSearch in Google Scholar
  • 32 Schnitzer E, Pinchuck I, Fainaru M, Schafer Z, Lichtenberg D. Copper-induced lipid oxidation in unfractioned plasma: The lag preceding oxidation as a measure of oxidation-resistance.  Biochem Biophys Res Com. 1995;  216 854-861
    PubMedSearch in Google Scholar
  • 33 Shern-Brewer R, Santanam N, Wetzstein C, White-Welkley J, Parthasarathy S. Exercise and cardiovascular disease: a new perspective.  Arterioscler Thromb Vasc Biol. 1998;  18 1181-1187
    PubMedSearch in Google Scholar
  • 34 Shimonov M, Pinchuk I, Bor A, Beigel I, Fainaru M, Rubin M, Lichtenberg D. Susceptibility of serum lipids to copper-induced peroxidation correlates with the level of high-density lipoprotein cholesterol.  Lipids. 1999;  34 255-259
    PubMedSearch in Google Scholar
  • 35 Sjodin B, Hellsten Y, Apple F S. Biochemical mechanisms for oxygen free radical formation during exercise.  Sport Med. 1990;  10 236-254
    CrossrefPubMedSearch in Google Scholar
  • 36 Vasankari T J, Kujala U M, Vasankari T M, Ahotupa M. Reduced oxidized LDL levels after a 10-month exercise program.  Med Sci Sports Exerc. 1998;  30 1496-1501
    PubMedSearch in Google Scholar
  • 37 Vasankari T J, Kujala U M, Vasankari T M, Vuorimaa T, Ahotupa M. Effects of acute prolonged exercise on serum and LDL oxidation and antioxidant defenses.  Free Rad Biol Med. 1997;  22 509-513
    CrossrefPubMedSearch in Google Scholar
  • 38 Vasankari T J, Kujala U M, Vasankari T M, Vuorimaa T, Ahotupa M. Increased serum and low-density-lipoprotein antioxidant potential after antioxidant supplementation in endurance athletes.  Am J Clin Nutr. 1997;  65 1052-1056
    PubMedSearch in Google Scholar
  • 39 Vincent K R, Vincent H K, Braith R W, Lennon S L, Lowenthal D T. Resistance exercise training attenuates exercise-induced lipid peroxidation in the elderly.  Eur J Appl Physiol. 2002;  87 416-423
    PubMedSearch in Google Scholar
  • 40 Wetzstein C J, Shern-Brewer R A, Santanam N, Green N R, White-Welkley J E, Parthasarathy S. Does acute exercise affect the susceptibility of low density lipoprotein to oxidation?.  Free Radic Biol Med. 1998;  24 679-682
    CrossrefPubMedSearch in Google Scholar

O. Inbar

Zinman College, Wingate Institute

Netania

Israel

Phone: + 97254531475

Fax: + 972 98 65 09 60

Email: inbar@macam.ac.il