Cytotoxicities of Xanthones and Cinnamate Esters from Hypericum hookerianum
Rujida Wilairat1, 2
, Jiradej Manosroi2
, Aranya Manosroi2
, Anake Kijjoa1, 3
, Maria São José Nascimento4
, Madalena Pinto5
, Artur M. S. Silva6
, Graham Eaton7
, Werner Herz8
1ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Porto, Portugal
2Department of Pharmaceutical Science, Faculty of Pharrmacy, Chiang Mai University, Chiang Mai, Thailand
3CIIMAR-Centro Interdisciplinar de Investigação Marítima e Ambiental, Universidade do Porto, Porto, Portugal
4Laboratório de Microbiologia, Faculdade de Farmácia e Centro de Estudos de Química Orgânica, Fitoquímica e Farmacologia da Universidade do Porto (CEQOFFUP), Universidade do Porto, Porto, Portugal
5Laboratório de Química Orgânica, Faculdade de Farmácia e Centro de Estudos de Química Orgânica, Fitoquímica e Farmacologia da Universidade do Porto (CEQOFFUP), Universidade do Porto, Porto, Portugal
6Department de Química, Universidade de Aveiro, Aveiro, Portugal
7Department of Chemistry, Leicester University, Leicester, UK
8Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL, USA
5-Hydroxy-2-methoxyxanthone (1), 2-hydroxy-3-methoxyxanthone (2), trans-kielcorin (3), 4-hydroxy-3-methoxyphenyl ferulate (4) and 3β-O-caffeoylbetulinic acid (5) were isolated from Hypericum hookerianum. Compounds 1 - 5 were tested against the growth of three human tumor cell lines, MCF-7, NCI-H460 and SF-268. Compounds 4 and 5 exhibited significant inhibitory activity effects against all three; GI50 values for 4 were 15.1 ± 1.6, 18.7 ± 2.3 and 15.9 ± 2.7 and for 5 12.2 ± 2.4, 19.6 ± 2.3 and 24.3 ± 2.5. Compound 3 was less active with GI50 values of 55.1 ± 2.3, 49.7 ± 3.0 and 40.5 ± 1.5, while 1 and 2 exhibited only weak effects. Compounds 4 and 5 were moderately effective in influencing the mitogenic response to human lymphocytes to hemoagglutinin, with IC values of 26.1 ± 3.6 and 40.8 ± 4.9, respectively.
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