Cytotoxicities of Xanthones and Cinnamate Esters from Hypericum hookerianum

Rujida Wilairat; Jiradej Manosroi; Aranya Manosroi; Anake Kijjoa; Maria São José Nascimento; Madalena Pinto; Artur M. S. Silva; Graham Eaton; Werner Herz

doi:10.1055/s-2005-871276

Planta Med 2005; 71(7): 680-682
DOI: 10.1055/s-2005-871276

Letter

Cytotoxicities of Xanthones and Cinnamate Esters from Hypericum hookerianum

Rujida Wilairat¹ ^, ² , Jiradej Manosroi² , Aranya Manosroi² , Anake Kijjoa¹ ^, ³ , Maria São José Nascimento⁴ , Madalena Pinto⁵ , Artur M. S. Silva⁶ , Graham Eaton⁷ , Werner Herz⁸

¹ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Porto, Portugal
²Department of Pharmaceutical Science, Faculty of Pharrmacy, Chiang Mai University, Chiang Mai, Thailand
³CIIMAR-Centro Interdisciplinar de Investigação Marítima e Ambiental, Universidade do Porto, Porto, Portugal
⁴Laboratório de Microbiologia, Faculdade de Farmácia e Centro de Estudos de Química Orgânica, Fitoquímica e Farmacologia da Universidade do Porto (CEQOFFUP), Universidade do Porto, Porto, Portugal
⁵Laboratório de Química Orgânica, Faculdade de Farmácia e Centro de Estudos de Química Orgânica, Fitoquímica e Farmacologia da Universidade do Porto (CEQOFFUP), Universidade do Porto, Porto, Portugal
⁶Department de Química, Universidade de Aveiro, Aveiro, Portugal
⁷Department of Chemistry, Leicester University, Leicester, UK
⁸Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL, USA

Abstract

5-Hydroxy-2-methoxyxanthone (1), 2-hydroxy-3-methoxyxanthone (2), trans-kielcorin (3), 4-hydroxy-3-methoxyphenyl ferulate (4) and 3β-O-caffeoylbetulinic acid (5) were isolated from Hypericum hookerianum. Compounds 1 - 5 were tested against the growth of three human tumor cell lines, MCF-7, NCI-H460 and SF-268. Compounds 4 and 5 exhibited significant inhibitory activity effects against all three; GI₅₀ values for 4 were 15.1 ± 1.6, 18.7 ± 2.3 and 15.9 ± 2.7 and for 5 12.2 ± 2.4, 19.6 ± 2.3 and 24.3 ± 2.5. Compound 3 was less active with GI₅₀ values of 55.1 ± 2.3, 49.7 ± 3.0 and 40.5 ± 1.5, while 1 and 2 exhibited only weak effects. Compounds 4 and 5 were moderately effective in influencing the mitogenic response to human lymphocytes to hemoagglutinin, with IC values of 26.1 ± 3.6 and 40.8 ± 4.9, respectively.

Full Text

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Prof. Werner Herz

Department of Chemistry and Biochemistry

Florida State University

Tallahassee

FL32306-4390

Fax: +1-850-644-8281

Email: jdulin@chem.fsu.edu