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DOI: 10.1055/s-2005-921157
Cyclodextrine zur Funktionalisierung von Textilien - In-vitro-Untersuchungen zur Hautverträglichkeit am Zellkulturmodell
Textile Finishing with Cyclodextrins - In Vitro Studies on the Skin Tolerability of Cyclodextrins Using a Cell Culture ModelPublikationsverlauf
Publikationsdatum:
14. Februar 2006 (online)
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Zusammenfassung
Anwendungen cyclodextrin-veredelter Textilien im dermatologischen oder kosmetischen Bereich machen eine Abklärung der Hautverträglichkeit der verwendeten Cyclodextrine notwendig. In der vorliegenden Arbeit wurde der Einfluss von unsubstituierten Cyclodextrinen und Cyclodextrin-Derivaten auf das Proliferationsverhalten epidermaler Zellen (HaCaT-Keratinozyten) untersucht. Während α- und γ-Cyclodextrine und ihre Derivate auch in hoher Konzentration (1 % w/v) gut von den Zellen toleriert werden, wirken β- und Methyl-β-Cyclodextrine dosisabhängig stark zytotoxisch. Diese Eigenschaften sollten beim In-vivo-Einsatz von Cyclodextrinen im dermatologischen Bereich unbedingt berücksichtigt werden.
Abstract
Applications of textiles finished with cyclodextrins for dermatological or cosmetic purposes require an assessment on the skin tolerability of the fixed cyclodextrins. In the present work, the influence of natural and chemically modified cyclodextrins on the proliferation behavior of a human epidermal cell line (HaCaT keratinocytes) was investigated. It was shown, that α- and γ-cyclodextrin were well tolerated by the cells even at high concentrations (1 % w/v). However, β- and methyl-β-cyclodextrin induce cytotoxic effects in a dose-dependent manner. For in vivo applications of β- and methyl-β-cyclodextrins these findings should be taken into consideration.
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Ute Schönfelder
Klinik für Dermatologie und dermatologische Allergologie · Friedrich-Schiller-Universität Jena
Erfurter Straße 35 · 07743 Jena
eMail: ute.schoenfelder@med.uni-jena.de