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Plant Biol (Stuttg) 2007; 9(2): 288-297
DOI: 10.1055/s-2006-924660
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Gas Exchange and Antioxidative Compounds in Young Beech Trees under Free-Air Ozone Exposure and Comparisons to Adult Trees

K. Herbinger1 , C. Then2 , 3 , K. Haberer4 , M. Alexou4 , M. Löw3 , K. Remele1 , H. Rennenberg4 , R. Matyssek3 , D. Grill1 , G. Wieser2 , M. Tausz1 , 5
  • 1Institut für Pflanzenwissenschaften, Universität Graz, Schuberstraße 51, 8010 Graz, Austria
  • 2Bundesamt und Forschungszentrum für Wald, Abteilung Forstpflanzenphysiologie, Rennweg 1, 6020 Innsbruck, Austria
  • 3Lehrstuhl für Ökophysiologie der Pflanzen, Technische Universität München, Life Science Center Weihenstephan, Am Hochanger 13, 85350 Freising, Germany
  • 4Institut für Forstbotanik und Baumphysiologie, Professur für Baumphysiologie, Albert-Ludwigs-Universität Freiburg, Georges-Köhler-Allee 053/054, 79085 Freiburg, Germany
  • 5School of Forest and Ecosystem Science, University of Melbourne, Water Street, Creswick, Victoria 3363, Australia
Further Information

Publication History

Received: April 28, 2006

Accepted: September 8, 2006

Publication Date:
13 March 2007 (online)

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Abstract

Three-year-old beech (Fagus sylvatica) seedlings growing in containers were placed into the sun and shade crown of a mature beech stand exposed to ambient (1 × O3) and double ambient (2 × O3) ozone concentrations at a free-air exposure system (“Kranzberg Forst”, Germany). Pigments, α-tocopherol, glutathione, ascorbate, and gas exchange were measured in leaves during 2003 (a drought year) and 2004 (an average year). Sun-exposed seedlings showed higher contents of antioxidants, xanthophylls, and β-carotene and lower contents of chlorophyll, α-carotene, and neoxanthin than shade-exposed seedlings. In 2003 sun-exposed seedlings showed higher contents of carotenoids and total glutathione and lower net photosynthesis rates (Amax) compared to 2004. O3 exposure generally affected the content of chlorophyll, the xanthophyll cycle, and the intercellular CO2 concentration (ci). Seedlings differed from the adjacent adult trees in most biochemical and physiological parameters investigated: Sun exposed seedlings showed higher contents of α-tocopherol and xanthophylls and lower contents of ascorbate, chlorophyll, neoxanthin, and α-carotene compared to adult trees. Shade exposed seedlings had lower contents of xanthophylls, α-carotene, and α-tocopherol than shade leaves of old-growth trees. In 2003, seedlings had higher Amax, stomatal conductance (gs), and ci under 2 × O3 than adult trees. The results showed that shade acclimated beech seedlings are more sensitive to O3, possibly due to a lower antioxidative capacity per O3 uptake. We conclude that beech seedlings are uncertain surrogates for adult beech trees.

Key words

Ozone - beech - Fagus sylvatica - tree age - antioxidants - pigments - leaf gas exchange.

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K. Herbinger

Institut für Pflanzenwissenschaften
Universität Graz

Schuberstraße 51

8010 Graz

Austria

Email: karin.herbinger@uni-graz.at

Editor: J. T. M. Elzenga

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