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Planta Med 1982; 46(9): 10-14
DOI: 10.1055/s-2007-970008
DOI: 10.1055/s-2007-970008
Research Articles
© Hippokrates Verlag Stuttgart
Immobilization of Strictosidine Synthase from Catharanthus Cell Cultures and Preparative Synthesis of Strictosidine
Further Information
Publication History
1982
Publication Date:
29 March 2007 (online)
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Abstract
Strictosidine synthase was partially purified from Catharanthus roseus cell suspension cultures and immobilized on CNBr-activated Sepharose. The immobilized enzyme exhibits a thermostability increased 300 fold over that of the soluble enzyme and catalyses exclusively the formation of the 3α(S)-isomer, strictosidine. Gram quantities of this biologically active glucoalkaloid, which hitherto had been difficult to synthesize and purify, were prepared.
Key Word Index
Catharanthus roseus - Apocynaceae - Cell Culture - Strictosidine Synthase - Immobilized Enzyme - Strictosidine Preparation