Planta Med 1982; 46(9): 10-14
DOI: 10.1055/s-2007-970008
Research Articles

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Immobilization of Strictosidine Synthase from Catharanthus Cell Cultures and Preparative Synthesis of Strictosidine

Ursula Pfitzner, M. H. Zenk
  • Lehrstuhl für Pharmazeutische Biologie, Universität München, Federal Republic of Germany
Further Information

Publication History

1982

Publication Date:
29 March 2007 (online)

Abstract

Strictosidine synthase was partially purified from Catharanthus roseus cell suspension cultures and immobilized on CNBr-activated Sepharose. The immobilized enzyme exhibits a thermostability increased 300 fold over that of the soluble enzyme and catalyses exclusively the formation of the 3α(S)-isomer, strictosidine. Gram quantities of this biologically active glucoalkaloid, which hitherto had been difficult to synthesize and purify, were prepared.