Abstract
Genome size determinations using propidium iodide flow cytometry were conducted in Lupinus anatolicus, L. pilosus, L. luteus, L. hispanicus ssp. hispanicus and L. hispanicus ssp. hispanicus × L. luteus (a complex hybridization product of L. hispanicus ssp. hispanicus and L. luteus). Using Clycine max “Ceresia” (1C = 1.134 pg) and an accession of L. pilosus (1C = 0.649 pg) as internal standards the following 1C values were obtained: L. anatolicus: 0.606 pg; L. pilosus: 0.620-0.678 pg; L. luteus: 1.173 pg; L. hispanicus: 1.043 pg; L. hispanicus ssp. hispanicus × L. luteus: 1.036 pg. Lupinus anatolicus is a newly described taxon with smooth seeds whose genome is approximately as small as the smallest genomes measured in the related L. pilosus, a taxon with rough seeds. The genomes in the related smooth-seeded taxa L. luteus, L. hispanicus and L. hispanicus ssp. hispanicus × L. luteus are considerably larger. There is a 1.13-fold difference between L. luteus (crop) and L. hispanicus (wild). The genome size of L. hispanicus ssp. hispanicus × L. luteus, however, is not intermediate, it is close to L. hispanicus.
Key words
Lupinus anatolicus
-
L. pilosus
-
L. luteus
-
L. hispanicus
-
L. hispanicus ssp. hispanicus × L. luteus
- genome size variation - flow cytometry
