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Horm Metab Res 1999; 31(2/03): 65-69
DOI: 10.1055/s-2007-978700
© Georg Thieme Verlag Stuttgart · New York

Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Insulin-Like Growth Factor I in Human Osteosarcoma MG-63 Cells

W. Lopaczynski, S. Harris, P. Nissley
  • National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
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1998

1998

Publication Date:
19 April 2007 (online)

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The activation of extracellular signal-regulated kinases 1 and 2 by insulin-like growth factor I in human osteosarcoma MG-63 cells was examined by Mono Q ion exchange chromatography of cell extracts and measurement of myelin basic protein kinase activity, and by immunoblotting of cell extracts with a phospho-specific extracellular signal-regulated kinase antibody. Extracellular signal-regulated kinase 1 appeared to be activated in resting cells and addition of insulin-like growth factor I resulted in the activation primarily of extracellular signal-regulated kinase 2. Extracellular signal-regulated kinase 2 was found in the nucleus after addition of insulin-like growth factor I.

Key words

Insulin-Like Growth Factor I Receptor - Mono Q Ion Exchange Chromatography - Nuclear Extracellular Signal Regulated Kinase 2