The Effects of Panduratin A Isolated from Kaempferia pandurata on the Expression of Matrix Metalloproteinase-1 and Type-1 Procollagen in Human Skin Fibroblasts

Abstract

Exposure of ultraviolet (UV) light on the skin induces photoaging associated with up-regulated matrix metalloproteinase (MMP) activities and decreased collagen synthesis. We investigated the effects of panduratin A isolated from Kaempferia pandurata Roxb. on the expression of matrix metalloproteinase-1 (MMP-1) and type-1 procollagen in UV-irradiated human skin fibroblasts. Cultured human fibroblasts were irradiated with UV (20 mJ/cm²) and panduratin A was added into the medium of the fibroblast culture. The expressions of MMP-1 and type-1 procollagen levels were measured using Western blot analysis and RT-RCR. Panduratin A in the range of 0.001 - 0.1 μM significantly reduced the expression of MMP-1 and induced the expression of type-1 procollagen at the protein and mRNA gene levels. Panduratin A showed stronger activity than epigallocatechin 3-O-gallate (EGCG) known as a natural anti-aging agent. The results suggest that panduratin A can be a potential candidate for the prevention and treatment of skin aging brought about by UV.

Abbreviations

UV:ultraviolet

MMP:matrix metalloproteinase

ROS:reactive oxygen species

DCFH-DA:2′,7′-dichlorofluorescein-diacetate

EGCG:epigallocatechin 3-O-gallate

RT-PCR:reverse transcriptase-polymerasechain reaction

Kaempferia pandurata - Zingiberaceae - panduratin A - UV irradiation - matrix metalloproteinase-1 - type-1 procollagen

References

• 1 Yaar M, Gilchrest B A. Cellular and molecular mechanisms of cutaneous aging.  J Dermatol Surg Oncol. 1990;  16 915-22
• 2 Saito Y, Shiga A, Yoshida Y, Furuhashi T, Fugita Y, Niki E. Effects of novel gaseous antioxidative system containing a rosemary extract on the oxidation induced by nitrogen dioxide and ultraviolet radiation.  Biosci Biotechnol Biochem. 2004;  68 781-6
• 3 Moon H I, Lee J K, Zee O P, Chung J H. A glycosidic isoflavonoid from Viola hondoensis W. Becker et H. Boissieu (Violaceae), and its effect on the expression of matrix metalloproteinase-1 caused by ultraviolet irradiation in cultured human skin fibroblasts.  Biol Pharm Bull. 2005;  28 1123-5
• 4 Fisher G J, Choi J C, Bata-Csorge Z, Shao Y, Subhash D, Wang Z Q. Ultraviolet irradiation increases matrix metalloproteinase-8 protein in human skin in vivo.  J Invest Dermatol. 2001;  117 219-26
• 5 Vincenti M P, Brinckerhoff C E. Transcriptional regulation of collagenase (MMP-1, MMP-13) genes in arthritis: Integration of complex signaling pathways for the recruitment of gene-specific transcription factors.  Arthritis Res. 2002;  4 157-64
• 6 Brenneisen P, Wenk J, Klotz L O, Wlaschek M, Briviba K, Krieg T. et al . Central role of ferrous/ferric iron in the ultraviolet B irradiation-mediated signaling pathway leading to increased interstitial collagenase (matrix-degrading metalloprotease (MMP)-1) and stromelysin-1 (MMP-3) mRNA levels in cultured human dermal fibroblasts.  J Biol Chem. 1998;  273 5279-87
• 7 Varani J, Warner R L, Gharaee-Kermani M, Phan S H, Kang S, Chung J H. et al . Vitamin A antagonizes decreased cell growth and elevated collagen-degrading matrix metalloproteinases and stimulates collagen accumulation in naturally aged human skin.  J Invest Dermatol. 2000;  114 480-6
• 8 Trakoontivakorn G, Nakahara K, Shinmoto H, Takenaka M, Onishi-Kameyama M, Ono H. et al . Structural analysis of a novel antimutagenic compound, 4-hydroxypanduratin A, and the antimutagenic activity of flavonoids in a Thai spice, fingerroot (Boesenbergia pandurata Schult.) against mutagenic heterocyclic amines.  J Agric Food Chem. 2001;  49 3046-50
• 9 Tuchinda P, Reutrakul V, Claeson P, Pongprayoon U, Sematong T, Santisuk T. et al . Anti-inflammatory cyclohexenyl chalcone derivatives in Boesenbergia pandurata.  Phytochemistry. 2002;  59 169-73
• 10 Calliste C A, Le Bail J C, Trouillas P, Pouget C, Habrioux G, Chulia A J. et al . Chalcones: Structural requirements for antioxidant, estrogenic and antiproliferative activities.  Anticancer Res. 2001;  21 3949-56
• 11 Mahidol C, Tantiwachwuttikul P, Reutrakul V, Taylor W C. Constituents of Boesenbergia pandurata (syn. Kaempferia pandurata). Isolation and synthesis of (±)-boesenbergin B.  Aust J Chem. 1984;  37 1739-45
• 12 Yun J M, Kweon M H, Kwon H J, Hwang J K, Mukhtar H. Induction of apoptosis and cell cycle arrest by a chalcone panduratin A isolated from Kaempferia pandurata in androgen-independent human prostate cancer cells PC3 and DU145.  Carcinogenesis. 2006;  27 1454-64
• 13 Park K M, Choo J H, Sohn J H, Lee S H, Hwang J K. Antibacterial activity of panduratin A isolated from Kaempferia pandurata against Porphyromonas gingivalis.  Food Sci Biotechnol. 2005;  14 286-9
• 14 Yun J M, Kwon H J, Hwang J K. In vitro anti-inflammatory activity of panduratin A isolated from Kaempferia pandurata in RAW264.7 cells.  Planta Med. 2003;  69 1102-8
• 15 Sohn J H, Han K L, Lee S H, Hwang J K. Protective effects of panduratin A against oxidative damage of tert-butylhydroperoxide in human HepG2 cells.  Biol Pharm Bull. 2005;  28 1083-6
• 16 Cheenpracha S, Karalai C, Ponglimanont C, Subhadhirasakul S, Tewtrakul S. Anti-HIV-1 protease activity of compounds from Boesenbergia pandurata.  Bioorg Med Chem. 2006;  14 1710-4
• 17 Kiat T S, Pippen R, Yusof R, Ibrahim H, Khalid N, Rahman N A. Inhibitory activity of cyclohexenyl chalcone derivatives and flavonoids of fingerroot, Boesenbergia rotunda (L.), toward dengue-2 virus NS3 protease.  Bioorg Med Chem Lett. 2006;  12 3337-40
• 18 Shindo K, Kato M, Kinoshita A, Kobayashi A, Koike Y. Analysis of antioxidant activities contained in the Boesenbergia pandurata Schult. Rhizome.  Biosci Biotechnol Biochem. 2006;  70 2281-4
• 19 Pandji C, Grimm C, Wray V, Witte L, Proksch P. Insecticidal constituents from four species of the Zingiberaceae.  Phytochemistry. 1993;  34 415-9
• 20 Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays.  J Immunol Methods. 1983;  65 55-63
• 21 Tobi S E, Gilbert M, Paul N, McMillan T J. The green tea polyphenol, epigallocatechin-3-gallate, protects against the oxidative cellular and genotoxic damage of UVA radiation.  Int J Cancer. 2002;  102 439-44
• 22 Jaipetch T, Reutrakul V, Tantiwachwuttikul P, Santisuk T. Flavonoids in the black rhizomes of Boesenbergia pandurata.  Phytochemistry. 1983;  22 625-6
• 23 Nerya O, Vaya J, Musa R, Izrael S. Glabrene and isoliquiritigenin as tyrosinase inhibitors from Licorice roots.  J Agric Food Chem. 2003;  51 1201-7
• 24 Vincenti M P, White L A, Schroen D J, Benbow U, Brinckerhoff C E. Regulating expression of the gene for matrix metalloproteinase-1 (collagenase): Mechanisms that control enzyme activity, transcription, and mRNA stability.  Crit Rev Eukaryot Gene Expr. 1996;  6 391-411
• 25 Fisher G J, Datta S C, Talwar H S, Wang Z Q, Varani J, Kang S. et al . Molecular basis of sun-induced premature skin aging and retinoid antagonism.  Nature. 1996;  379 335-9
• 26 Lim H, Kim H P. Inhibition of mammalian collagenase, matrix metalloproteinase-1, by naturally-occurring flavonoids.  Planta Med. 2007;  73 1267-74
• 27 Demeule M, Brossard M, Page M, Gingras D, Beliveau R. Matrix metalloproteinase inhibition by green tea catechins.  Biochem Biophys Acta. 2000;  1478 51-60
• 28 Stetler-Stevenson W G, Yu A E. Proteases in invasion: Matrix metalloproteinase.  Sem Cancer Biol. 2001;  11 143-52
• 29 Robinson M J, Cobb M H. Mitogen-activated protein kinase pathways.  Curr Opin Cell Biol. 1997;  9 180-6