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Horm Metab Res 2008; 40(4): 262-268
DOI: 10.1055/s-2008-1058063
Animals, Clinical

© Georg Thieme Verlag KG Stuttgart · New York

A Combination of CLA-DAG Oil Modifies the Diabetic Phenotype in Male Zucker Diabetic Fatty Rats

M. J. Iqbal 1 [*] , A. Higginbotham 1 , N. Chickris 1 , M. Bollaert 1 , S. Rockway 2 , W. J. Banz 1
  • 1Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, Illinois, USA
  • 2Department of Clinical Nutrition, Rush University Medical Center, Chicago, Illinois, USA
Further Information

Publication History

received 22.01.2007

accepted 07.09.2007

Publication Date:
13 March 2008 (online)

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Abstract

A number of human and animal studies using conjugated linoleic acids (CLA) or diacylglycerol (DAG) oil have shown positive physiological effects on abdominal adiposity, plasma triglycerides, plasma glucose, and insulin sensitivity. A novel DAG composition containing CLA called CLA diacylglyceride (CLA-DAG) may offer potential as a therapeutic agent in reducing some of the symptoms associated with the diabetic phenotype and metabolic syndrome. This study was designed to investigate the effect of CLA-DAG oil on the diabetic phenotype in male Zucker diabetic fatty rats. Animals were assigned to one of four groups: control (C), rosiglitazone (ROS), CLA-DAG, or CLA as free fatty acid (CLA-FFA). After 11 weeks, body weight was higher and kidney weight was lower in the CLA-DAG and ROS groups compared with the C group. The ROS treatment increased the percentage of body fat as compared with all other groups. Final fasting blood glucose was lower in the CLA-DAG and ROS groups than in the C group. Plasma cholesterol was lower in the CLA-DAG group, and plasma triglycerides were lower in the ROS group compared with the C group. We also observed changes in transcript abundance of PPAR-γ, PPAR-α, FAS, LPL, UCP2, UCP3, CPT1, RxR, ObRb, ApoAII, ApoD, and IRS1 in liver, muscle, and adipose tissue, suggesting treatment-induced effects on these genes. Collectively, these data suggest the need for further research on the therapeutic relevance of CLA-DAG oil in obesity and diabetes. Future research should also differentiate between CLA alone and DAG alone compared with the combination.

Key words

transcript abundance - liver - muscle tissue - adipose tissue - metabolic syndrome

References

  • 1 Taylor CG, Zahradka P. Dietary conjugated linoleic acid and insulin sensitivity and resistance in rodent models.  Am J Clin Nutr. 2004;  79 1164S-1168S
    PubMedSearch in Google Scholar
  • 2 Nagao K, Yanagita T. Conjugated fatty acids in food and their health benefits.  J Biosci Bioeng. 2005;  100 152-157
    CrossrefPubMedSearch in Google Scholar
  • 3 Zulet MA, Marti A, Parra MD, Martinez JA. Inflammation and conjugated linoleic acid: mechanisms of action and implications for human health.  J Physiol Biochem. 2005;  61 483-494
    PubMedSearch in Google Scholar
  • 4 Tricon S, Burdge GC, Jones EL, Russell JJ, El Khazen S, Moretti E, Hall WL, Gerry AB, Leake DS, Grimble RF, Williams CM, Calder PC, Yaqoob P. Effects of dairy products naturally enriched with cis-9, trans-11 conjugated linoleic acid on the blood lipid profile in healthy middle-aged men.  Am J Clin Nutr. 2006;  83 744-753
    PubMedSearch in Google Scholar
  • 5 Toomey S, MacMonagle J, Roche HM. Conjugated linoleic acid: a functional nutrient in the different pathophysiological components of the metabolic syndrome?.  Curr Opin Clin Nutr Metab Care. 2006;  9 740-747
    PubMedSearch in Google Scholar
  • 6 Wahle KW, Heys SD, Rotondo D. Conjugated linoleic acids: are they beneficial or detrimental to health?.  Prog Lipid Res. 2004;  43 553-587
    PubMedSearch in Google Scholar
  • 7 Flickinger BD, Matsuo N. Nutritional characteristics of DAG oil.  Lipids. 2003;  38 129-132
    CrossrefPubMedSearch in Google Scholar
  • 8 Rudkowska I, Roynette CE, Demonty I, Vanstone CA, Jew S, Jones PJ. Diacylglycerol: efficacy and mechanism of action of an anti-obesity agent.  Obes Res. 2005;  13 1864-1876
    PubMedSearch in Google Scholar
  • 9 Takase H, Shoji K, Hase T, Tokimitsu I. Effect of diacylglycerol on postprandial lipid metabolism in non-diabetic subjects with and without insulin resistance.  Atherosclerosis. 2005;  180 197-204
    CrossrefPubMedSearch in Google Scholar
  • 10 Nagao K, Inoue N, Wang YM, Yanagita T. Conjugated linoleic acid enhances plasma adiponectin level and alleviates hyperinsulinemia and hypertension in Zucker diabetic fatty (fa/fa) rats.  Biochem Biophys Res Commun. 2003;  310 562-566
    CrossrefPubMedSearch in Google Scholar
  • 11 Wendel AA, Belury MA. Effects of conjugated linoleic acid and troglitazone on lipid accumulation and composition in lean and Zucker diabetic fatty (fa/fa) rats.  Lipids. 2006;  41 241-247
    CrossrefPubMedSearch in Google Scholar
  • 12 Nehlin JO, Mogensen JP, Petterson I, Jeppesen L, Fleckner J, Wulff EM, Sauerberg P. Selective PPAR agonists for the treatment of type 2 diabetes.  Ann NY Acad Sci. 2006;  1067 448-453
    CrossrefPubMedSearch in Google Scholar
  • 13 Ramachandran U, Kumar R, Mittal A. Fine tuning of PPAR ligands for type 2 diabetes and metabolic syndrome.  Mini Rev Med Chem. 2006;  6 563-573
    CrossrefPubMedSearch in Google Scholar
  • 14 Zhou X, Sun C, Jiang L, Wang H. Effect of conjugated linoleic acid on PPAR gamma gene expression and serum leptin in obese rat.  Wei Sheng Yan Jiu. 2004;  33 307-309
    PubMedSearch in Google Scholar
  • 15 Kang K, Liu W, Albright KJ, Park Y, Pariza MW. Trans-10,cis-12 CLA inhibits differentiation of 3T3-L1 adipocytes and decreases PPAR gamma expression.  Biochem Biophys Res Commun. 2003;  303 795-799
    CrossrefPubMedSearch in Google Scholar
  • 16 Moya-Camarena SY, Vanden Heuvel JP, Blanchard SG, Leesnitzer LA, Belury MA. Conjugated linoleic acid is a potent naturally occurring ligand and activator of PPARalpha.  J Lipid Res. 1999;  40 1426-1433
    PubMedSearch in Google Scholar
  • 17 Davis J, Iqbal MJ, Steinle J, Oitker J, Higginbotham DA, Peterson RG, Banz WJ. Soy protein influences the development of the metabolic syndrome in male obese ZDFxSHHF rats.  Horm Metab Res. 2005;  37 316-325
    Thieme ConnectPubMedSearch in Google Scholar
  • 18 Davis J, Steinle J, Higginbotham DA, Oitker J, Peterson RG, Banz WJ. Soy protein influences insulin sensitivity and cardiovascular risk in male lean SHHF rats.  Horm Metab Res. 2005;  37 309-315
    Thieme ConnectPubMedSearch in Google Scholar
  • 19 Reeves PG, Nielsen FH, Fahey  Jr  GC. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet.  J Nutr. 1993;  123 1939-1951
    PubMedSearch in Google Scholar
  • 20 Bookout AL, Mangelsdorf DJ. Quantitative real-time PCR protocol for analysis of nuclear receptor signaling pathways.  Nucl Recept Signal. 2003;  1 e012
    PubMedSearch in Google Scholar
  • 21 Banz WJ, Davis J, Peterson R, Iqbal MJ. Gene expression and adiposity are modified by soy protein in male Zucker diabetic fatty rats.  Obes Res. 2004;  12 1907-1913
    PubMedSearch in Google Scholar
  • 22 Belury MA, Mahon A, Banni S. The conjugated linoleic acid (CLA) isomer, t10c12-CLA, is inversely associated with changes in body weight and serum leptin in subjects with type 2 diabetes mellitus.  J Nutr. 2003;  133 257S-260S
    PubMedSearch in Google Scholar
  • 23 Shimokawa T, Kato M, Watanabe Y, Hirayama R, Kurosaki E, Shikama H, Hashimoto S. In vivo effects of pioglitazone on uncoupling protein-2 and -3 mRNA levels in skeletal muscle of hyperglycemic KK mice.  Biochem Biophys Res Commun. 1998;  251 374-378
    CrossrefPubMedSearch in Google Scholar
  • 24 Rassart E, Bedirian A, Do CS, Guinard O, Sirois J, Terrisse L, Milne R. Apolipoprotein D.  Biochim Biophys Acta. 2000;  1482 185-198
    PubMedSearch in Google Scholar
  • 25 Wang Y, Jones PJ. Dietary conjugated linoleic acid and body composition.  Am J Clin Nutr. 2004;  79 1153S-1158S
    PubMedSearch in Google Scholar
  • 26 Ryder JW, Portocarrero CP, Song XM, Cui L, Yu M, Combatsiaris T, Galuska D, Bauman DE, Barbano DM, Charron MJ, Zierath JR, Houseknecht KL. Isomer-specific antidiabetic properties of conjugated linoleic acid. Improved glucose tolerance, skeletal muscle insulin action, and UCP-2 gene expression.  Diabetes. 2001;  50 1149-1157
    PubMedSearch in Google Scholar
  • 27 Song J, Knepper MA, Hu X, Verbalis JG, Ecelbarger CA. Rosiglitazone activates renal sodium- and water-reabsorptive pathways and lowers blood pressure in normal rats.  J Pharmacol Exp Ther. 2004;  308 426-433
    PubMedSearch in Google Scholar

1 Current address: The Institute for Sustainable and Renewable Resources, Institute for Advanced Learning and Research; and Department of Horticulture at Virginia Polytechnic Institute and State University, 150 Slayton Avenue, Danville, Virginia 24540; Email: mjiqbal@ialr.org

Correspondence

W.J. BanzPhD, RD 

Department of Animal Science, Food and Nutrition

Southern Illinois University

62901-4317 Carbondale

Illinois

USA

Phone: +1/618/453 75 11

Fax: +1/618/453 75 17

Email: banz@siu.edu