RSS-Feed abonnieren
DOI: 10.1160/TH03-10-0644
Moderate-intensity exercise suppresses platelet activation and polymorphonuclear leukocyte interaction with surface-adherent platelets under shear flow in men
Financial support The study was support under the National Science Council Grant NSC 92-2314-B-182-021.Publikationsverlauf
Received
23. Oktober 2003
Accepted after revision
04. Januar 2003
Publikationsdatum:
05. Dezember 2017 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/200403/lookinside/thumbnails/10-1160-th03-10-0644-1.jpg)
Summary
The reciprocal modulation of platelet and polymorphonuclear leukocyte (PMN) activities is important in the pathogenesis of thrombosis and inflammation. This study investigated how moderate exercise affects shear-induced platelet activation and subsequent PMNs interaction with platelet-related thrombi under shear flow. Sixteen sedentary healthy men engaged in moderate exercise (about 60% V·O2max) on a bicycle ergometer. Platelet activation, PMNs interaction with surface-adherent platelets, and PMN-dependent inhibition of platelet activation under shear flow were measured both before and immediately after exercise. The results of this study can be summarized as follows: (1) moderate exercise was associated with lower extents of shear-induced platelet adhesion and aggregation, binding of von Willbrand factor (vWF) to platelets, and glycoprotein IIb/IIIa activation and P-selectin expression on platelet than at rest; (2) the velocity and percentage of rolling PMNs increased while the number of PMNs remaining bound to surface-adherent platelets decreased after moderate exercise; (3) although treating the PMNs with oxidized-low density lipoprotein (Ox-LDL) enhanced PMNs interaction with surface-adherent platelets, moderate exercise suppressed the enhancement of platelet-PMN interaction by Ox-LDL; (4) moderate exercise decreased platelet [Ca2+]i elevation induced by ADP and platelet [Ca2+]i levels mediated by PMN and Ox-LDL-treated PMN; and (5) plasma and PMN-derived nitric oxide metabolites and plasma vWF antigen and activity increased after moderate exercise, whereas plasma and platelet-derived soluble P-selectin levels remained unchanged in response to exercise. Therefore, we conclude that moderate-intensity exercise suppresses shear-induced platelet activation and subsequent PMNs adhesion to platelets deposited at sites of vascular injury under flow, thereby reducing the risks of vascular thrombosis and inflammation.
-
References
- 1 Wang JS, Jen CJ, Chen HI. Effects of exercise training and deconditioning on platelet function in men. Arterioscler Thromb Vasc Biol 1995; 15: 1668-74.
- 2 Wang JS, Jen CJ, Chen HI. Effects of chronic exercise and deconditioning on platelet function in women. J Appl Physiol 1997; 83: 2080-5.
- 3 Arraiz GA, Wigle DT, Mao Y. Risk assessment of physical activity and physical fitness in the Canada Health Survey mortality followup study. J Clin Epidemiol 1992; 45: 419-28.
- 4 Konstantopoulos K, Grotta JC, Sills C. et al. Shear-induced platelet aggregation in normal subjects and stroke patients. Thromb Haemost 1995; 74: 1329-34.
- 5 Neumann F-J, Marx N, Gawaz M. et al. Induction of cytokine expression in leukocytes by binding of thrombin-stimulated platelets. Circulation 1996; 94: 2387-94.
- 6 Bazzoni G, Dejana E, Del Maschio A. Platelet-neutrophil interactions possible relevance in the pathogenesis of thrombosis and inflammation. Haematologica 1991; 76: 491-9.
- 7 Mickelson JK, Lakkis NM. Villarreal-Levy et al. Leukocyte activation with platelet adhesion after coronary angioplasty: a mechanism for recurrent disease?. J Am Coll Cardiol 1996; 28: 345-53.
- 8 Hagberg IA, Roald HE, Lyberg T. Adhesion of leukocytes to growing arterial thrombosis. Thromb Haemost 1998; 80: 852-8.
- 9 Zatta A, Prosdocimi M, Bazzoni G. et al. Inhibition of platelet function by polymorphonuclear leukocytes. J Lab Clin Med 1990; 116: 651-60.
- 10 Faint RW, Mackie IJ, Machin SJ. Platelet aggregation is inhibited by a nitric oxide-like factor released from human neutrophils in vitro. Br J Haematol 1991; 71: 539-45.
- 11 Wang JS, Jen CJ, Kung HC. et al. Different effects of strenuous exercise and moderate exercise on platelet function in men. Circulation 1994; 90: 2877-85.
- 12 Wang JS, Jen CJ, Lee H. et al. Effects of shortterm exercise on female platelet function during different phases of the menstrual cycle. Arterioscler Thromb Vasc Biol 1997; 17: 1682-6.
- 13 Pedersen BK, Nielsen HB. Acute exercise and the immune system. In Pedersen BK. (ed). Exercise Immunology. Austin, Texas: Landes Company; 1997: 5-32.
- 14 Wang JS, Chow SE, Chen JK. Strenuous, acute exercise affects reciprocal modulation of platelet and polymorphonuclear leukocyte activities under shear flow in men. J Thromb Haemost 2003; 01: 2031-7.
- 15 Li N, Wallen NH, Hjemdahl P. Evidence for prothrombotic effects of exercise and limited protection by aspirin. Circulation 1999; 100: 1374-9.
- 16 Hilberg T, Schmidt V, Glaser D. et al. Platelet activity, sensitivity to agonist, and plateletleukocyte conjugate formation after long-term exercise. Platelets 2002; 13: 273-7.
- 17 Hilberg T, Glaser D, Schmidt V. et al. Shortterm exercise and platelet activity, sensitivity to agonist, and platelet-leukocyte conjugate formation. Platelets 2003; 14: 67-74.
- 18 Wang JS, Cheng L-J. The effect of strenuous acute exercise on α2-adrenergic agonist-potentiated platelet activation. Arterioscler Thromb Vasc Biol 1999; 19: 1559-65.
- 19 Konstantopoulos K, Chow TW, Turner NA. et al. Shear stress-induced binding of von Willebrand factor to platelet. Biorheology 1997; 34: 57-71.
- 20 Diacovo TG, Roth SJ, Buccola JM. et al. Neutrophil rolling, arrest, and transmigration across activated, surface-adherent platelets via sequential action of P-selectin and the β2-integrin CD11b/CD18. Blood 1996; 88: 146-57.
- 21 Wang JS, Chow S-E, Chen J-K. et al. Effect of exercise training on oxidized LDL-mediated platelet function in rats. Thromb Haemost 2000; 83: 503-8.
- 22 Green Lc, Wagner DA, Glogowski J. et al. Analysis of nitrate, nitrite and [15N]nitrate in biological fluids. Anal Biochem 1982; 126: 131-8.
- 23 Lee KW, Lip GY. Effects of lifestyle on hemostasis, fibrinolysis, and platelet reactivity: a systematic review. Arch Inter Med 2003; 163: 2368-92.
- 24 Siscovick DS, Weiss NS, Fletcher RH. et al. The incidence of primary cardiac arrest during vigorous exercise. N Engl J Med 1984; 311: 987-7.
- 25 El-Sayed MS, Sale C, Jones PGW. et al. Blood hemostasis in exercise and training. Med Sci Sports Exerc 2000; 32: 918-25.
- 26 Holme PA, Orvim U, Hamers MJ. et al. Shearinduced platelet activation and platelet microparticle formation at blood flow conditions as in arteries with a severe stenosis. Arterioscler Thromb Vasc Biol 1997; 17: 646-53.
- 27 Goto S, Salomon DR, Ikeda Y. et al. Characterization of the unique mechanism mediating the shear-dependent binding of soluble von Willebrand factor to platelets. J Biol Chem 1995; 270: 23352-61.
- 28 Ikeda Y, Handa M, Kawano K. et al. The role of von Willebrand factor and fibrinogen in platelet aggregation under varying shear stress. J Clin Invest 1991; 87: 1234-40.
- 29 Beumer S, Heijnen HFG, Ijsseldijk MJW. et al. Platelet adhesion to fibronectin in flow: The importance of von Willebrand factor and glycoprotein Ib. Blood 1995; 86: 3452-60.
- 30 Beumer S, Heijnen HFG, Ijsseldijk MJW. et al. Platelet adhesion to fibronectin in flow: The importance of von Willebrand factor and glycoprotein Ib. Blood 1995; 86: 3452-60.
- 31 Dong J-F, Bernat MC, Schade A. et al. Ristocetin-dependent, but not botrocetindependent, binding of von Willebrand factor to the platelet glycoprotein Ib-IX-V complex correlates with shear-dependent interactions. Blood 2001; 97: 162-8.
- 32 Depraetere H, Ajzenberg N, Girma J-P. et al. Platelet aggregation induced by a monoclonal antibody to A1 Domain of von Willebrand factor. Blood 1998; 91: 3792-9.
- 33 Chow TW, Hellums JD, Moake JL. et al. Shear stress-induced von Willebrand factor binding to platelet glycoprotein Ib initials calcium influx associated with aggregation. Blood 1992; 80: 113-20.
- 34 Stenberg PE, McEver RP, Shuman MA. et al. A platelet alpha-granule membrance protein (GMP 140) is expressed to plasma membrane after activation. J Cell Biol 1985; 101: 880-6.
- 35 Spangenberg P, Redlich H, Bergmann I. et al. The platelet glycoprotein IIb/IIIa complex is involved in the adhesion of activation activated platelets to leukocytes. Thromb Haemost 1993; 70: 514-21.
- 36 Kaul S, Makkar RR, Nakamura M. et al. Inhibition of acute stent thrombosis under high-shear flow conditions by a nitric oxide donor, DMHD/NO. An ex vivo porcine arteriovenous shunt study. Circulation 1996; 94: 2228-32.
- 37 Michelson AD, Benoit SE, Furman MI. et al. Effects of nitric oxide/EDRF on platelet surface glycoproteins. Am J Physiol 1996; 270: H1640-8.
- 38 Jenkins RR. Free radical chemistry. Relationship exercise. Sports Med 1988; 156-70.
- 39 Cooke JP, Tsao PS. Is NO an endogenous antiatherogenic molecule?. Arterioscl Thromb 1994; 14: 653-5.