Mice deficient in tissue factor demonstrate attenuated intimal hyperplasia in response to vascular injury and decreased smooth muscle cell migration

Robert T. Pyo; Yuichiro Sato; Nigel Mackman; Mark B. Taubman

doi:10.1160/TH04-02-0122

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2004; 92(03): 451-458
DOI: 10.1160/TH04-02-0122

Theme Issue Article

Schattauer GmbH

Mice deficient in tissue factor demonstrate attenuated intimal hyperplasia in response to vascular injury and decreased smooth muscle cell migration

Authors

Robert T. Pyo

¹The Cardiovascular Institute and Department of Medicine, Mount Sinai Medical Center, New York, New York
Yuichiro Sato

²Department of Immunology, The Scripps Research Institute, La Jolla, California
Nigel Mackman

²Department of Immunology, The Scripps Research Institute, La Jolla, California
Mark B. Taubman

¹The Cardiovascular Institute and Department of Medicine, Mount Sinai Medical Center, New York, New York

³Department of Medicine, University of Rochester, Rochester, New York, USA

Abstract

Summary

Tissue factor (TF) is the primary initiator of the coagulation cascade and is thought to play a key role in the generation of arterial thrombosis. Recent studies have suggested that TF mediates inflammatory processes in the arterial wall and may be an important regulator of intimal hyperplasia. We have employed genetically engineered mice (mTF^−/−/hTF⁺) with markedly diminished TF activity (≈1% normal levels) to examine the role of TF in mediating the response to arterial injury. mTF^−/−/hTF⁺ displayed a marked reduction in intimal hyperplasia (46% decrease in intimal area, 60% decrease in intimal/medial ratio) in response to femoral artery injury when compared to wild type controls. The decreased intimal hyperplasia seen in low TF mice was noted in a model of vascular injury not associated with significant thrombosis, suggesting that it may be mediated by non-procoagulant properties of TF. Smooth muscle cells from mTF^−/−/hTF⁺ mice grew normally in response to serum, but exhibited a marked defect in cell migration in a modified Boyden chamber assay. In contrast, there was no difference in platelet derived growth factorinduced migration, suggesting that the effect of TF on smooth muscle cell migration is agonist dependent. These data suggest that TF may mediate intimal hyperplasia by regulating smooth muscle cell migration.

Keywords

Vascular smooth muscle - cell migration - arterial injury - genetically altered mice - tissue factor

Full Text

References

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