A subpopulation of human B lymphocytes can express a functional Tissue Factor in response to phorbol myristate acetate

Houria Mechiche; Pascale Cornillet-Lefebvre; Philippe Nguyen

doi:10.1160/TH04-12-0845

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2005; 94(01): 146-154
DOI: 10.1160/TH04-12-0845

Platelets and Blood Cells

Schattauer GmbH

A subpopulation of human B lymphocytes can express a functional Tissue Factor in response to phorbol myristate acetate

Houria Mechiche

¹Laboratoire Central d’Hématologie, CHU de Reims, and UPRES EA 3796, IFR53, Faculté de Médecine et de Pharmacie, Reims, France

,

Pascale Cornillet-Lefebvre

¹Laboratoire Central d’Hématologie, CHU de Reims, and UPRES EA 3796, IFR53, Faculté de Médecine et de Pharmacie, Reims, France

,

Philippe Nguyen

¹Laboratoire Central d’Hématologie, CHU de Reims, and UPRES EA 3796, IFR53, Faculté de Médecine et de Pharmacie, Reims, France

› Author Affiliations

Abstract

Summary

Tissue Factor (TF, CD142) is a transmembrane glycoprotein that belongs to the cytokine receptor superfamily. This multifunctional protein is constitutively present in most tissues. Among circulating blood cells, TF expression is known to be restricted to monocytes which do not constitutively produce TF but express TF in response to various stimuli. Here, we report that highly purified B lymphocytes can support a procoagulant activity (PCA) in response to phorbol myristate acetate (PMA).Using flow cytometry (FCM), we observed that a subpopulation of B lymphocytes (CD19⁺) can expressTF and anionic phospholipids in response to PMA. TF protein was identified and characterized by immunofluorescence, immunocytochemistry and electronic microscopy. Using RT-PCR, we identified the presence of TF mRNA in response to PMA. In conclusion, B lymphocytes are a potential source of functional TF in human.

Keywords

Tissue factor - procoagulant activity - B lymphocyte - PMA

Full Text

References

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