The antigenic binding sites of autoantibodies to factor XII in patients with recurrent pregnancy losses

Akifumi Inomo; Toshitaka Sugi; Yoshimi Fujita; Hidehiko Matsubayashi; Shun-ichiro Izumi; Mikio Mikami

doi:10.1160/TH07-07-0447

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2008; 99(02): 316-323
DOI: 10.1160/TH07-07-0447

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

The antigenic binding sites of autoantibodies to factor XII in patients with recurrent pregnancy losses

Akifumi Inomo^*

¹Department of Obstetrics and Gynecology, Specialized Clinical Science, Tokai University School of Medicine, Kanagawa, Japan

,

Toshitaka Sugi^*

¹Department of Obstetrics and Gynecology, Specialized Clinical Science, Tokai University School of Medicine, Kanagawa, Japan

,

Yoshimi Fujita

¹Department of Obstetrics and Gynecology, Specialized Clinical Science, Tokai University School of Medicine, Kanagawa, Japan

,

Hidehiko Matsubayashi

¹Department of Obstetrics and Gynecology, Specialized Clinical Science, Tokai University School of Medicine, Kanagawa, Japan

,

Shun-ichiro Izumi

¹Department of Obstetrics and Gynecology, Specialized Clinical Science, Tokai University School of Medicine, Kanagawa, Japan

,

Mikio Mikami

¹Department of Obstetrics and Gynecology, Specialized Clinical Science, Tokai University School of Medicine, Kanagawa, Japan

› Author Affiliations

Abstract

Summary

Recently, numerous studies have suggested an association between factor XII (FXII) deficiency and recurrent pregnancy losses, and between autoantibodies to FXII and recurrent pregnancy losses. Autoantibodies to FXII rather than FXII deficiency may be a risk factor for recurrent pregnancy losses. To know the pathogenesis of autoantibodies to FXII, epitope mapping study was done. Seventeen anti-FXII antibody positive recurrent pregnancy loss patients were chosen for this study. We used synthetic peptides in inhibition and direct binding studies to identify the antigenic binding site of autoantibodies to FXII. Among plasmas from 17 recurrent pregnancy loss patients who were positive for autoantibodies to FXII, 13 patients (76.5%) recognized amino acids 1–30, the amino-terminal heavy chain region that is known as factor XII binding site to platelet glycoprotein Ibα.

Keywords

Recurrent pregnancy losses - factor XII - kininogen - kallikreinkinin system - antiphosphatidylethanolamine antibody - platelet

Full Text

References

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