Contribution of (sub)domains of Staphylococcus aureus fibronectin-binding protein to the proinflammatory and procoagulant response of human vascular endothelial cells

Ruth Heying; Joke van de Gevel; Yok-Ai Que; Lionel Piroth; Philippe Moreillon; Henry Beekhuizen

doi:10.1160/TH08-06-0395

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2009; 101(03): 495-504
DOI: 10.1160/TH08-06-0395

Wound Healing and Inflammation/Infection

Schattauer GmbH

Contribution of (sub)domains of Staphylococcus aureus fibronectin-binding protein to the proinflammatory and procoagulant response of human vascular endothelial cells

Ruth Heying

¹Department of Paediatric Cardiology, University Children‘s Hospital, Duesseldorf, Germany

²Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands

,

Joke van de Gevel

²Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands

,

Yok-Ai Que

³Department of Critical Care Medicine, CHUV and University of Lausanne, Lausanne, Switzerland

,

Lionel Piroth

⁴Infectious Diseases Department, University Hospital, Dijon, France

,

Philippe Moreillon

⁵Institutes of Fundamental Microbiology, UNIL, Lausanne, Switzerland

,

Henry Beekhuizen

²Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands

› Institutsangaben

Abstract

Summary

The Staphylococcus aureus fibronectin (Fn) -binding protein A (FnBPA) is involved in bacterium-endothelium interactions which is one of the crucial events leading to infective endocarditis (IE). We previously showed that the sole expression of S. aureus FnBPA was sufficient to confer to non-invasive Lactococcus lactis bacteria the capacity to invade human endothelial cells (ECs) and to launch the typical endothelial proinflammatory and procoagulant responses that characterize IE. In the present study we further questioned whether these bacterium-EC interactions could be reproduced by single or combined FnBPA subdomains (A, B, C or D) using a large library of truncated FnBPA constructs expressed in L. lactis. Significant invasion of cultured ECs was found for L. lactis expressing the FnBPA subdomains CD (aa 604–877) or A4⁺¹⁶ (aa 432–559). Moreover, this correlates with the capacity of these fragments to elicit in vitro a marked increase in EC surface expression of both ICAM-1 and VCAM-1 and secretion of the CXCL8 chemokine and finally to induce a tissue factor-dependent endothelial coagulation response. We thus conclude that (sub)domains of the staphylococcal FnBPA molecule that express Fn-binding modules, alone or in combination, are sufficient to evoke an endothelial proinflammatory as well as a procoagulant response and thus account for IE severity.

Keywords

Bacterial infection - endothelial cells - inflammation - thrombosis - tissue factor / factor VII

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Referenzen

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