Confirmatory procedure and other maneuvers to assess pathogenicity of platelet factor 4 (PF4)-dependent antibodies – distinguishing

Theodore E. Warkentin

doi:10.1160/TH08-08-0533

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2008; 100(04): 523-524
DOI: 10.1160/TH08-08-0533

Editorial Focus

Schattauer GmbH

Confirmatory procedure and other maneuvers to assess pathogenicity of platelet factor 4 (PF4)-dependent antibodies – distinguishing "signal" from "noise"

Theodore E. Warkentin

¹Department of Pathology and Molecular Medicine, and Department of Medicine

²Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada

› Author Affiliations

Abstract

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References

References
1 Amiral J, Bridey F, Dreyfus M. et al. Platelet factor 4 complexed to heparin is the target for antibodies generated in heparin-induced thrombocy-topenia. Thromb Haemost 1992; 68: 95-96.
2 Warkentin TE, Sheppard JI, Horsewood P. et al. Impact of the patient population on the risk for heparin-induced thrombocytopenia. Blood 2000; 96: 1703-1708.
3 Warkentin TE, Sheppard JI, Moore JC. et al. Laboratory testing for the antibodies that cause heparin-induced thrombocytopenia: how much class do we need?. J Lab Clin Med 2005; 146: 341-346.
4 Lo GK, Sigouin CS, Warkentin TE. What is the potential for overdiagnosis of heparin-induced thrombocytopenia?. Am J Hematol 2007; 82: 1037-1043.
5 Package insert, 'PF4 Enhanced®', GTI, Waukesha, WI, January 13, 2005.
6 Warkentin TE, Sheppard JI. No significant improvement in diagnostic specificity of an anti-PF4/polyanion immunoassay with use of high heparin confirmatory procedure. J Thromb Haemost 2006; 04: 281-282.
7 Whitlatch NL, Perry SL, Ortel TL. Anti-heparin/ platelet factor 4 antibody optical density values and the confirmatory procedure in the diagnosis of heparin induced thrombocytopenia. Thromb Haemost 2008; 100: 678-684.
8 Moore JC, Arnold DM, Warkentin TE. et al. An algorithm for resolving 'indeterminate' test results in the platelet serotonin release assay for investigation of heparin-induced thrombocytopenia. J Thromb Haemost 2008; 06: 1595-1597.
9 Zwicker JI, Uhl L, Huang WY. et al. Thrombosis and ELISA optical density values in hospitalized patients with heparin-induced thrombocytopenia. J Thromb Haemost 2004; 02: 2133-2137.
10 Warkentin TE, Sheppard JI, Moore JC. et al. Quantitative interpretation of optical density measurements using PF4-dependent enzyme-immunoassays. J Thromb Haemost 2008; 06: 1304-1312.