The potential for circular dichroism as an additional facile and sensitive method of monitoring low-molecular-weight heparins and heparinoids

Timothy R. Rudd; Mark A. Skidmore; Scott E. Guimond; Joseph Holman; Jeremy E. Turnbull; Robert M. Lauder; David G. Fernig; Edwin A. Yates

doi:10.1160/TH08-12-0797

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2009; 102(05): 874-878
DOI: 10.1160/TH08-12-0797

Theme Issue Article

Schattauer GmbH

The potential for circular dichroism as an additional facile and sensitive method of monitoring low-molecular-weight heparins and heparinoids

Timothy R. Rudd^*

¹School of Biological Sciences, University of Liverpool, Liverpool, UK

,

Mark A. Skidmore^*

¹School of Biological Sciences, University of Liverpool, Liverpool, UK

,

Scott E. Guimond

¹School of Biological Sciences, University of Liverpool, Liverpool, UK

,

Joseph Holman

¹School of Biological Sciences, University of Liverpool, Liverpool, UK

,

Jeremy E. Turnbull

¹School of Biological Sciences, University of Liverpool, Liverpool, UK

,

Robert M. Lauder

²School of Health and Medicine, Division of Biomedical and Life Sciences, Lancaster University, Bailrigg, Lancaster, UK

,

David G. Fernig

¹School of Biological Sciences, University of Liverpool, Liverpool, UK

,

Edwin A. Yates

¹School of Biological Sciences, University of Liverpool, Liverpool, UK

› Author Affiliations

Abstract

Summary

The ultraviolet circular dichroism (CD) spectra of commercial low-molecular-weight heparins, heparinoids and other anticoagulant preparations have been recorded between 180 and 260 nm. Principal component analysis of the spectra allowed their differentiation into a number of groups related to the means of their production reflecting the structural changes introduced by each process. The findings suggest that CD provides a complementary technique for the rapid analysis of heparin preparations.

Keywords

Circular dichroism - low-molecular-weight heparin - heparinoids - principal component analysis - quality control

Full Text

References

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