Subscribe to RSS
![](/products/assets/desktop/img/oa-logo.png)
DOI: 10.4103/1305-7456.119074
Effect of mixing techniques on bacterial attachment and disinfection time of polyether impression material
Publication History
Publication Date:
25 September 2019 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00042133/2013S01/lookinside/thumbnails/10-4103-1305-7456-119074_00s54-1.jpg)
ABSTRACT
Objective: The aim of this study was 2-fold. The first aim was to evaluate the effects of mixing technique (hand-mixing or auto-mixing) on bacterial attachment to polyether impression materials. The second aim was to determine whether bacterial attachment to these materials was affected by length of exposure to disinfection solutions. Materials and Methods: Polyether impression material samples (n = 144) were prepared by hand-mixing or auto-mixing. Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were used in testing. After incubation, the bacterial colonies were counted and then disinfectant solution was applied. The effect of disinfection solution was evaluated just after the polymerization of impression material and 30 min after polymerization. Differences in adherence of bacteria to the samples prepared by hand-mixing and to those prepared by auto-mixing were assessed by Kruskal-Wallis and Mann-Whitney U-tests. For evaluating the efficiency of the disinfectant, Kruskal-Wallis multiple comparisons test was used. Results: E. coli counts were higher in hand-mixed materials (p < 0.05); no other statistically significant differences were found between hand- and auto-mixed materials. According to the Kruskal-Wallis test, significant differences were found between the disinfection procedures (Z > 2.394). Conclusions: The methods used for mixing polyether impression material did not affect bacterial attachment to impression surfaces. In contrast, the disinfection procedure greatly affects decontamination of the impression surface.
-
REFERENCES
- 1 Memarian M, Fazeli MR, Jamalifar H, Azimnejad A. Disinfection efficiency of irreversible hydrocolloid impressions using different concentrations of sodium hypochlorite: A pilot study. J Contemp Dent Pract 2007; 8: 27-34
- 2 Schutt RW. Bactericidal effect of a disinfectant dental stone on irreversible hydrocolloid impressions and stone casts. J Prosthet Dent 1989; 62: 605-7
- 3 Wu G, Yu X, Gu Z. Ultrasonically nebulised electrolysed oxidising water: A promising new infection control programme for impressions, metals and gypsum casts used in dental hospitals. J Hosp Infect 2008; 68: 348-54
- 4 Ribeiro DG, Pavarina AC, Dovigo LN, Palomari Spolidorio DM, Giampaolo ET, Vergani CE. Denture disinfection by microwave irradiation: A randomized clinical study. J Dent 2009; 37: 666-72
- 5 Egusa H, Watamoto T, Matsumoto T, Abe K, Kobayashi M, Akashi Y. et al. Clinical evaluation of the efficacy of removing microorganisms to disinfect patient-derived dental impressions. Int J Prosthodont 2008; 21: 531-8
- 6 Egusa H, Watamoto T, Abe K, Kobayashi M, Kaneda Y, Ashida S. et al. An analysis of the persistent presence of opportunistic pathogens on patient-derived dental impressions and gypsum casts. Int J Prosthodont 2008; 21: 62-8
- 7 Al-Jabrah O, Al-Shumailan Y, Al-Rashdan M. Antimicrobial effect of 4 disinfectants on alginate, polyether, and polyvinyl siloxane impression materials. Int J Prosthodont 2007; 20: 299-307
- 8 Drennon DG, Johnson GH, Powell GL. The accuracy and efficacy of disinfection by spray atomization on elastomeric impressions. J Prosthet Dent 1989; 62: 468-75
- 9 Flanagan DA, Palenik CJ, Setcos JC, Miller CH. Antimicrobial activities of dental impression materials. Dent Mater 1998; 14: 399-404
- 10 Tobias RS, Browne RM, Wilson CA. An in vitro study of the antibacterial and antifungal properties of an irreversible hydrocolloid impression material impregnated with disinfectant. J Prosthet Dent 1989; 62: 601-5
- 11 Schwartz RS, Hensley DH, Bradley Jr DV. Immersion disinfection of irreversible hydrocolloid impression in pH-adjusted sodium hypochlorite. Part 1: Microbiology. Int J Prosthodont 1996; 9: 217-22
- 12 Shilinburg HT, Hobo S, Whitsett LD, Jacobi R, Brackett SE. Fundamentals of Fixed Prosthodontics. 3 rd ed. Canada: Quintessence Pub; 1997: p. 302-5
- 13 Lepe X, Johnson GH, Berg JC, Aw TC. Effect of mixing technique on surface characteristics of impression materials. J Prosthet Dent 1998; 79: 495-502
- 14 Wang J, Wan Q, Chao Y, Chen Y. A self-disinfecting irreversible hydrocolloid impression material mixed with chlorhexidine solution. Angle Orthod 2007; 77: 894-900
- 15 Hutchings ML, Vandewalle KS, Schwartz RS, Charlton DG. Immersion disinfection of irreversible hydrocolloid impressions in pH-adjusted sodium hypochlorite. Part 2: Effect on gypsum casts. Int J Prosthodont 1996; 9: 223-9
- 16 Melilli D, Rallo A, Cassaro A, Pizzo G. The effect of immersion disinfection procedures on dimensional stability of two elastomeric impression materials. J Oral Sci 2008; 50: 441-6
- 17 Samaranayake LP, Hunjan M, Jennings KJ. Carriage of oral flora on irreversible hydrocolloid and elastomeric impression materials. J Prosthet Dent 1991; 65: 244-9
- 18 Beyerle MP, Hensley DM, Bradley Jr DV, Schwartz RS, Hilton TJ. Immersion disinfection of irreversible hydrocolloid impressions with sodium hypochlorite. Part I: Microbiology. Int J Prosthodont 1994; 7: 234-8
- 19 McNeill MR, Coulter WA, Hussey DL. Disinfection of irreversible hydrocolloid impressions: A comparative study. Int J Prosthodont 1992; 5: 563-7
- 20 Bergman B. Disinfection of prosthodontic impression materials: A literature review. Int J Prosthodont 1989; 2: 537-42
- 21 Taylor RL, Wright PS, Maryan C. Disinfection procedures: Their effect on the dimensional accuracy and surface quality of irreversible hydrocolloid impression materials and gypsum casts. Dent Mater 2002; 18: 103-10
- 22 Walker MP, Rondeau M, Petrie C, Tasca A, Williams K. Surface quality and long-term dimensional stability of current elastomeric impression materials after disinfection. J Prosthodont 2007; 16: 343-51
- 23 Jagger DC, Vowles RW, McNally L, Davis F, O›Sullivan DJ. The effect of a range of disinfectants on the dimensional accuracy and stability of some impression materials. Eur J Prosthodont Restor Dent 2007; 15: 23-8
- 24 Bal BT, Yilmaz H, Aydin C, Yilmaz C, Al FD. Antibacterial and antifungal properties of polyether impression materials. J Oral Sci 2007; 49: 265-70
- 25 Ten Broeke-Smits NJ, Kummer JA, Bleys RL, Fluit AC, Boel CH. Hair follicles as a niche of Staphylococcus aureus in the nose; is a more effective decolonisation strategy needed?. J Hosp Infect 2010; 76: 211-4
- 26 Liguori G, Bagattini M, Gallè F, Quartucci V, Di Onofrio V, Negrone M. et al. Microbiological evaluation of the efficacy of two new biodetergents on multidrug-resistant nosocomial pathogens. Ann Clin Microbiol Antimicrob 2009; 8: 35
- 27 Uneke CJ, Ogbonna A, Oyibo PG, Onu CM. Bacterial contamination of stethoscopes used by health workers: Public health implications. J Infect Dev Ctries 2010; 4: 436-41