Acute Exercise Increases the Expression of KIR2DS4 by Promoter Demethylation in NK Cells

Alexander Schenk; Walter Pulverer; Christine Koliamitra; Claus Juergen Bauer; Suzana Ilic; Rudolf Heer; Robert Schier; Volker Schick; Bernd W. Böttiger; Clarissa Gerhäuser; Wilhelm Bloch; Philipp Zimmer

doi:10.1055/a-0741-7001

International Journal of Sports Medicine, Inhaltsverzeichnis

Int J Sports Med 2019; 40(01): 62-70
DOI: 10.1055/a-0741-7001

Genetics & Molecular Biology

Acute Exercise Increases the Expression of KIR2DS4 by Promoter Demethylation in NK Cells

Alexander Schenk

¹Department of molecular and cellular sport medicine, Institute of cardiovascular research and sports medicine; German Sport University Cologne, Cologne, Germany

,

Walter Pulverer

²Department Health and Environment, AIT Austrian Institute of Technology GmbH, Vienna, Austria

,

Christine Koliamitra

¹Department of molecular and cellular sport medicine, Institute of cardiovascular research and sports medicine; German Sport University Cologne, Cologne, Germany

,

Claus Juergen Bauer

³Department of Anesthesiology and Intesive Care medicine, University Hospital Cologne, Cologne, Germany

,

Suzana Ilic

²Department Health and Environment, AIT Austrian Institute of Technology GmbH, Vienna, Austria

,

Rudolf Heer

²Department Health and Environment, AIT Austrian Institute of Technology GmbH, Vienna, Austria

,

Robert Schier

³Department of Anesthesiology and Intesive Care medicine, University Hospital Cologne, Cologne, Germany

,

Volker Schick

³Department of Anesthesiology and Intesive Care medicine, University Hospital Cologne, Cologne, Germany

,

Bernd W. Böttiger

³Department of Anesthesiology and Intesive Care medicine, University Hospital Cologne, Cologne, Germany

,

Clarissa Gerhäuser

⁴Deutsches Krebsforschungszentrum, Epigenomics and Cancer Risk Factors, Heidelberg, Germany

,

Wilhelm Bloch

¹Department of molecular and cellular sport medicine, Institute of cardiovascular research and sports medicine; German Sport University Cologne, Cologne, Germany

,

Philipp Zimmer

¹Department of molecular and cellular sport medicine, Institute of cardiovascular research and sports medicine; German Sport University Cologne, Cologne, Germany

⁵Division of Physical Activity, Prevention and Cancer, German Cancer Research Center (DKFZ) and National Center for Tumor Diseases (NCT), Heidelberg, Germany

› Institutsangaben

Abstract

Positive effects of exercise on cancer prevention and progression have been proposed to be mediated by stimulating natural killer (NK) cells. Because NK cell receptors are regulated by epigenetic modifications, we investigated whether acute aerobic exercise and training change promoter DNA methylation and gene expression of the activating KIR2DS4 and the inhibiting KIR3DL1 gene. Sixteen healthy women (50–60 years) performed a graded exercise test (GXT) and were randomized into either a passive control group or an intervention group performing a four-week endurance exercise intervention. Blood samples (pre-, post-GXT and post-training) were used for isolation of DNA/RNA of NK cells to assess DNA promoter methylation by targeted deep-amplicon sequencing and gene expression by qRT-PCR. Potential changes in NK cell subsets were determined by flow cytometry. Acute and chronic exercise did not provoke significant alterations of NK cell proportions. Promoter methylation decreased and gene expression increased for KIR2DS4 after acute exercise. A high gene expression correlated with a low methylation of CpGs that were altered by acute exercise. Chronic exercise resulted in a minor decrease of DNA methylation and did not alter gene expression. Acute exercise provokes epigenetic modifications, affecting the balance between the activating KIR2DS4 and the inhibiting KIR3DL1, with potential benefits on NK cell function.

Key words

physical activity - exercise - natural killer cells - NK cells - epigenetic - DNA methylation

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Referenzen

References
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