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Planta Med 2019; 85(11/12): 911-916
DOI: 10.1055/a-0877-7255
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Development and Validation of a UHPLC-DAD Method for the Quantitative Analysis of Major Dihydrochalcone Glucosides from Thonningia sanguinea VAHL[*]

Luca Pompermaier
1   Institute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Innsbruck, Austria
,
Stefan Schwaiger
1   Institute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Innsbruck, Austria
,
Monizi Mawunu
2   Kimpa Vita University, Province of Uíge, Uíge, Angola
,
Thea Lautenschlaeger
3   Department of Biology, Institute of Botany, Faculty of Science, Technische Universität Dresden, Dresden, Germany
,
Hermann Stuppner
1   Institute of Pharmacy/Pharmacognosy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Innsbruck, Austria
› Author Affiliations
Further Information

Publication History

received 30 January 2019
revised 05 March 2019

accepted 11 March 2019

Publication Date:
22 March 2019 (online)

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Abstract

Thonnigia sanguinea is a plant widely used in traditional African medicine against a variety of diseases. The obligate parasite is growing throughout tropical African forests and utilizes a large variety of hosts. Dihydrochalcone glucoside derivatives isolated from the subaerial parts of this plant were identified as potential antidiabetic lead compounds. In this study, an ultrahigh-performance liquid chromatographic method coupled with a photodiode array detector was developed for the quantitation of six major dihydrochalcone derivatives. The analytes were baseline separated in complex samples within 14 minutes on a Phenomenex Luna Omega 1.6 µm C18 column using a mobile phase consisting of water and acetonitrile (each + 0.01% trifluoroacetic acid) in gradient elution. Method validation confirmed the selectivity, linearity (R2 ≥ 0.9992), precision (inter-day ≤ 1.98%, intraday ≤ 2.00%), and accuracy (recovery rates of 97.4 – 106.3% for all analytes). At 280 nm, the LODs and LOQs were found to be lower than 1.42 and 4.30 µg/mL, respectively. Eight plant batches from the northern Angolan province of Uíge (collected in the wild or bought on markets) were extracted with methanol using an ultrasound-assisted extraction protocol and subsequently analyzed with the validated method. Results indicated high contents of dihydrochalcone glucosides in all eight samples. Most notably, the two bioactive constituents thonningianin A and B were present in fairly large amounts (2.42 – 5.35 w%).

Key words

Thonningia sanguinea - Balanophoraceae - UHPLC-DAD - dihydrochalcone glucosides - thonningianin A - thonningianin B

* Dedicated to Professor Dr. Cosimo Pizza in recognition of his outstanding contribution to phytotherapy research.


 

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