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Planta Med 2019; 85(17): 1374-1382
DOI: 10.1055/a-1023-8940
DOI: 10.1055/a-1023-8940
Biological and Pharmacological Activity
Original Papers
Alkaloid Enantiomers from Isatis tinctoria with Neuroprotective Effects against H2O2-Induced SH-SY5Y Cell Injury
Supported by: National Natural Science Foundation of China 81973462 Supported by: China Postdoctoral Science Foundation 2016M593031 Supported by: China Postdoctoral Science Foundation 2018T111135 Supported by: Natural Science Foundation of Liaoning Province of China 2019-MS-303 Supported by: Career Development Support Plan for Young, Middle-aged Teachers in Shenyang Pharmaceutical University ZQN2018006
Further Information
Publication History
received 29 August 2019
revised 28 September 2019
accepted 06 October 2019
Publication Date:
04 November 2019 (online)
Abstract
Six pairs of alkaloid enantiomers including 11 new alkaloids (1a/1b–5a/5b, 6a) were isolated from the leaves of Isatis tinctoria. Their structures were established by extensive spectroscopic analyses. Enantiomers were separated successfully by chiral chromatographic column and the absolute configurations of all isolates were determined by comparison of experimental and calculated electronic circular dichroism spectra. The isolated alkaloids were evaluated for their neuroprotective activities against H2O2-induced cell injury in human neuroblastoma SH-SY5Y cells. The results showed that 5a/5b and 6a/6b exhibited potent neuroprotective activities at 50 µM compared with the H2O2-treated group.
Supporting Information
- Supporting Information
NMR, HRESIMS, UV, ECD spectrum, chiral HPLC chromatograms, and further detailed experimental information are available as Supporting Information.
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