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Planta Med 2020; 86(07): 470-481
DOI: 10.1055/a-1120-3711
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Comparative Morpho-Anatomical and HPTLC Profiling of Tinospora Species and Dietary Supplements

Abidah Parveen
1   Division of Pharmacognosy, Department of Biomolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, USA
2   National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
3   Department of Pharmaceutical Sciences, Abbottabad University of Science and Technology, Havelian, KPK, Pakistan
,
John S. Adams
2   National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
,
Vijayasankar Raman
2   National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
,
Jane M. Budel
4   Department of Pharmaceutical Sciences, State University of Ponta Grossa (UEPG), Ponta Grossa, PR, Brazil
,
Jianping Zhao
2   National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
,
Ganesh N.M. Babu
5   The University of Trans-disciplinary Health Sciences and Technology, Bengaluru, Karnataka, India
,
Zulfiqar Ali
2   National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
,
Ikhlas A. Khan
1   Division of Pharmacognosy, Department of Biomolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, USA
2   National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
› Author AffiliationsSupported by: U.S. Food and Drug Administration 5U01FD004246-07
Further Information

Publication History

received 15 November 2019
revised 16 January 2020

accepted 14 February 2020

Publication Date:
13 March 2020 (online)

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Abstract

Overlapping geographical occurrence, history of traditional use, confusion in species identification, and morphological resemblances among various species are some considerations that necessitate the importance of qualitative analysis for efficient quality control and safer botanical products. This paper provides detailed morpho-anatomies of the leaves and stems of Tinospora cordifolia, Tinospora crispa, and Tinospora sinensis, and stems of Tinospora baenzigeri. Microscopy studies of the selected Tinospora species revealed key diagnostic features that can help distinguish the closely related species of Tinospora as well as to detect any adulteration or substitution in the raw materials. HPTLC profiles of the authenticated plant materials, as well as commercial products claiming to contain Tinospora, were compared to distinguish T. crispa from other closely related species and to establish an efficient method to assess the identity and quality of the products using qualified chemical markers. HPTLC chromatograms of both plant samples and dietary supplements were compared with six reference marker compounds. The analysis revealed that borapetoside B and C were useful to identify T. crispa while tinosineside A was found to be characteristic to authenticate the T. sinensis products.

Key words

Tinospora - Menispermaceae - anatomy - HPTLC - microscopy - quality control
 

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