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Planta Med 2020; 86(07): 489-495
DOI: 10.1055/a-1127-7503
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Evaluation of the hepatotoxic potential of Tinospora crispa and its isolated borapetosides B, C and F in a murine model

Abidah Parveen
1   Division of Pharmacognosy, Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, USA
2   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, USA
3   Department of Pharmaceutical Sciences, Abbottabad University of Science and Technology, Havelian, KPK, Pakistan
,
Mir Tahir Maqbool
2   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, USA
,
Yan-Hong Wang
2   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, USA
,
Zulfiqar Ali
2   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, USA
,
Ikhlas A. Khan
1   Division of Pharmacognosy, Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, USA
2   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, USA
,
M. Khalid Ashfaq
2   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, USA
› Author Affiliations
Supported by: U.S. Department of Agriculture 58-6060-6-015 Supported by: U.S. Food and Drug Administration 2U01FD004246-06
Further Information

Publication History

received 08 December 2019
revised 25 February 2020

accepted 26 February 2020

Publication Date:
26 March 2020 (online)

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Abstract

The hepatotoxic potential of the methanolic extract of the stems of Tinospora crispa and of its furano-diterpenoid–borapetosides B, C, and F–was investigated in normal and health-compromised mice. Health-compromised condition was established by a single intraperitoneal injection of LPS (6 mg/kg). Two different sets of experiments were conducted to evaluate the hepatotoxic potential. A 21-day study where the mice were dosed with the extract of T. crispa (1 gm/kg b. wt./day) or standardized combination of borapetosides B, C and F (500 mg/kg b. wt.) with or without a single dose of LPS (1 gm/kg b. wt./day). In the acute toxicity study, mice were dosed with borapetosides B, C, and F (500 mg/kg b. wt.). Results showed that the ALT levels were normal and liver histopathology unaltered. No conclusive hepatotoxicity was observed in the methanolic extract or pure compounds tested under the given experimental conditions.

Key words

Tinospora crispa - Menispermaceae - borapetoside F - hepatotoxicity - lipopolysaccharide - alanine transaminase