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Am J Perinatol 2024; 41(S 01): e212-e220
DOI: 10.1055/a-1877-6306
Original Article

Effect of Routine Gastric Residual Aspiration on the Preterm Infant Fecal Microbiome

Mary W. Lenfestey
1   Department of Pediatrics, Pediatric Gastroenterology, East Carolina University, Greenville, North Carolina
,
Nan Li
2   Department of Pediatrics, Division of Neonatology, University of Florida, Gainesville, Florida
,
Josee Gauthier
3   Division of Gastroenterology, Department of Medicine, University of Florida, Gainesville, Florida
,
Kathryn Winglee
4   Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, North Carolina
,
Anthony Fodor
4   Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, North Carolina
,
Ke Zeng
4   Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, North Carolina
,
Christian Jobin
5   Department of Medicine, Gastroenterology, University of Florida, Gainesville, Florida
,
Josef Neu
6   Department of Pediatrics, Neonatology, University of Florida, Gainesville, Florida
,
Leslie A. Parker
7   College of Nursing, University of Florida, Gainesville, Florida
› Author Affiliations Funding This work was supported by the National Institute of Nursing Research. U.S. Department of Health and Human Services, National Institutes of Health, National Institute of Nursing Research, grant no.: R01DK088244.
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Abstract

Objective Enteral feeding tubes are used in neonatal intensive care units (NICUs) to assess feeding tolerance by utilizing preprandial gastric residual aspiration. This study evaluates the effect of gastric residual aspiration on the preterm infant fecal microbiome and gastrointestinal inflammation.

Study Design Fifty-one very low birth weight (VLBW) infants (≤32 weeks' gestational age and ≤1,250 g) enrolled in a larger single-center randomized controlled trial evaluating the effects of routine and nonroutine gastric residual aspiration were selected for further analysis. Of those infants, 30 had microbiome analysis performed on stools collected at 6 weeks by sequencing the bacterial V1 to V3 variable regions of the genes encoding for 16S rRNA. In an additional 21 infants, stool samples collected at 3 and 6 weeks were analyzed for intestinal inflammation using a cytokine multiplex panel.

Results Microbial communities between groups were not distinct from each other and there was no difference in intestinal inflammation between groups. Analyses using gene expression packages DESeq2 and edgeR produced statistically significant differences in several taxa, possibly indicating a more commensal intestinal microbiome in infants not undergoing gastric residual aspiration.

Conclusion Omission of routine gastric residual aspiration was not associated with intestinal dysbiosis or inflammation, providing additional evidence that monitors preprandial gastric residuals is unnecessary.

Key Points

  • Omission of routine gastric residual aspiration was not associated with intestinal dysbiosis or inflammation.

  • Existing literature indicates preprandial gastric aspiration does not reliably correlate with development of necrotizing enterocolitis but does correlate with delayed enteral nutrition.

  • Further study is required but this data that suggest monitoring preprandial gastric residuals are unnecessary.

Keywords

neonate - gastric residual - gastric aspiration - microbiome

Authors' Contributions

Substantial contributions to the conception and design, acquisition of data, or analysis and interpretation of data: M.W.L., N.L., J.G., K.W., A.F., K.Z., C.J., J.N., and L.A.P.


-Drafting the article or revising it critically for important intellectual content: M.W.L., N.L., L.A.P., and J.N.


-Final approval of the version to be published: J.G., K.W., A.F., K.Z., and C.J.


Consent Statement

A member of the research team obtained informed parental consent for participation in the study for each enrolled infant.


Ethical Approval

The Institutional Review Board at the University of Florida reviewed and approved the study protocol.


Note

This study is registered with ClincalTrials.gov with identifier: NCT01863043.


Supplementary Material



Publication History

Received: 31 January 2022

Accepted: 09 June 2022

Accepted Manuscript online:
16 June 2022

Article published online:
22 August 2022

© 2022. Thieme. All rights reserved.

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