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Facial Plast Surg 2023; 39(02): 148-154
DOI: 10.1055/a-1910-0748
Original Research

The Effect of Melatonin on Nasal Septal Wound Healing in an Experimental Animal Model

Doğan Çakan 
1   ENT Department, Cerrrahpasa Medicine Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey
,
Semih Uşaklıoğlu 
2   ENT Department, University of Health Sciences Istanbul Haseki Training and Research Hospital, Istanbul, Turkey
› Institutsangaben Funding None.
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Abstract

The purpose of our study was to examine the effect of melatonin (MLT) on wound healing in the nasal septum. Twenty-two Sprague–Dawley rats of the male sex were included in this experimental study. Nasal septal perforation (NSP), about 2 mm in diameter, was formed in each rat. MLT was applied topically to the subjects in the study group and saline was applied topically to the subjects in the control group once a day for 14 days. On day 14, the rats were sacrificed and the nasal septums of the subjects were resected for pathological evaluation. In the NSP area, degeneration and regeneration of nasal septal epithelium; degeneration and regeneration of the septal cartilage; number of cells involved in wound healing such as acute inflammatory cells (leukocytes), fibroblast, eosinophil, and giant cell; capillary vessel intensity; granulation tissue formation; and collagen intensity parameters were evaluated histopathologically. The macroscopic size and histopathologic examination results of NSPs were statistically analyzed. In the MLT group, the epithelium regeneration, cartilage regeneration, fibroblast number, collagen density, vascularity, and granulation formation were significantly higher, and the epithelial degeneration and acute inflammatory cells density were significantly lower, in the NSP area (p < 0.05). In addition, macroscopic healing was significantly higher in the MLT group (p = 0.044 and <0.05). Local application of MLT accelerates nasal septal wound healing. With this feature, the usage of MLT can be promising to prevent the formation of NSP.

Keywords

animals - melatonin - wound healing - nasal septal perforation - rats

Note

The present study was confirmed by Bezmialem University Ethics Committee for Animal Experiments (date/no: February 22, 2021/33) and performed at Bezmialem University Research Center. All institutional and national guidelines for the care and use of laboratory animals were followed. Each of the author has contributed in reviewing and approving this manuscript. The experiment was done by D.Ç. The article was written by D.Ç. and S.U. This manuscript is original and it, or any part of it, has not been previously published, nor is it under consideration for publication elsewhere.




Publikationsverlauf

Accepted Manuscript online:
26. Juli 2022

Artikel online veröffentlicht:
03. Dezember 2022

© 2022. Thieme. All rights reserved.

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  • References

  • 1 Lindley LE, Stojadinovic O, Pastar I, Tomic-Canic M. Biology and Biomarkers for Wound Healing. Plast Reconstr Surg 2016; 138 (3, suppl): 18S-28S
    Google Scholar
  • 2 Alobid I. Endoscopic approach for management of septal perforation. Eur Arch Otorhinolaryngol 2019; 276 (08) 2115-2123
    Google Scholar
  • 3 Gold M, Boyack I, Caputo N, Pearlman A. Imaging prevalence of nasal septal perforation in an urban population. Clin Imaging 2017; 43 (43) 80-82
    Google Scholar
  • 4 Pereira C, Santamaría A, Langdon C, López-Chacón M, Hernández-Rodríguez J, Alobid I. Nasoseptal perforation: from etiology to treatment. Curr Allergy Asthma Rep 2018; 18 (01) 5
    Google Scholar
  • 5 Kridel RWH, Delaney SW. Simultaneous septal perforation repair with septorhinoplasty: a 31-year experience. Facial Plast Surg 2018; 34 (03) 298-311
    Google Scholar
  • 6 Kridel RWH, Delaney SW. Approach to correction of septal perforation. Facial Plast Surg Clin North Am 2019; 27 (04) 443-449
    Google Scholar
  • 7 Çakan D, Aydın S, Demir G, Başak K. The effect of curcumin on healing in an animal nasal septal perforation model. Laryngoscope 2019; 129 (10) E349-E354
    Google Scholar
  • 8 Kasuya A, Tokura Y. Attempts to accelerate wound healing. J Dermatol Sci 2014; 76 (03) 169-172
    Google Scholar
  • 9 Acuña-Castroviejo D, Escames G, Venegas C. et al. Extrapineal melatonin: sources, regulation, and potential functions. Cell Mol Life Sci 2014; 71 (16) 2997-3025
    CrossrefPubMedGoogle Scholar
  • 10 Zhang S, Chen S, Li Y, Liu Y. Melatonin as a promising agent of regulating stem cell biology and its application in disease therapy. Pharmacol Res 2017; 117: 252-260
    CrossrefPubMedGoogle Scholar
  • 11 Kaczmarek-Szczepańska B, Ostrowska J, Kozłowska J. et al. Evaluation of polymeric matrix loaded with melatonin for wound dressing. Int J Mol Sci 2021; 22 (11) 5658
    CrossrefPubMedGoogle Scholar
  • 12 Ozler M, Simsek K, Ozkan C. et al. Comparison of the effect of topical and systemic melatonin administration on delayed wound healing in rats that underwent pinealectomy. Scand J Clin Lab Invest 2010; 70 (06) 447-452
    CrossrefPubMedGoogle Scholar
  • 13 Ceylan SM, Uysal E, Sokucu M. et al. The effects of Halofuginone on wound healing in the rat nasal mucosa. Am J Rhinol Allergy 2020; 34 (01) 9-15
    Google Scholar
  • 14 Pugazhenthi K, Kapoor M, Clarkson AN, Hall I, Appleton I. Melatonin accelerates the process of wound repair in full-thickness incisional wounds. J Pineal Res 2008; 44 (04) 387-396
    CrossrefPubMedGoogle Scholar
  • 15 Faul F, Erdfelder E, Lang A-G, Buchner A. G*Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods 2007; 39 (02) 175-191
    Google Scholar
  • 16 Santamaría-Gadea A, Mariño-Sánchez F, Arana-Fernández B, Mullol J, Alobid I. Innovative surgical techniques for nasal septal perforations: management and treatment. Curr Allergy Asthma Rep 2021; 21 (03) 17
    Google Scholar
  • 17 Wang PH, Huang BS, Horng HC, Yeh CC, Chen YJ. Wound healing. J Chin Med Assoc 2018; 81 (02) 94-101
    Google Scholar
  • 18 Murali R, Thanikaivelan P, Cheirmadurai K. Melatonin in functionalized biomimetic constructs promotes rapid tissue regeneration in Wistar albino rats. J Mater Chem B Mater Biol Med 2016; 4 (35) 5850-5862
    Google Scholar
  • 19 Jin H, Zhang Z, Wang C. et al. Melatonin protects endothelial progenitor cells against AGE-induced apoptosis via autophagy flux stimulation and promotes wound healing in diabetic mice. Exp Mol Med 2018; 50 (11) 1-15
    CrossrefPubMedGoogle Scholar
  • 20 Lee SJ, Jung YH, Oh SY, Yun SP, Han HJ. Melatonin enhances the human mesenchymal stem cells motility via melatonin receptor 2 coupling with Gαq in skin wound healing. J Pineal Res 2014; 57 (04) 393-407
    CrossrefPubMedGoogle Scholar
  • 21 Mukai K, Nakajima Y, Asano K, Nakatani T. Topical estrogen application to wounds promotes delayed cutaneous wound healing in 80-week-old female mice. PLoS One 2019; 14 (11) e0225880
    Google Scholar
  • 22 Ozawa S, Mukudai S, Sugiyama Y, Branski RC, Hirano S. Mechanisms underlying the antifibrotic potential of estradiol for vocal fold fibrosis. Laryngoscope 2021; 131 (10) 2285-2291
    Google Scholar
  • 23 Buscemi N, Vandermeer B, Hooton N. et al. Efficacy and safety of exogenous melatonin for secondary sleep disorders and sleep disorders accompanying sleep restriction: meta-analysis. BMJ 2006; 332 (7538): 385-393
    CrossrefPubMedGoogle Scholar
  • 24 Lopes Rocha Correa V, Assis Martins J, Ribeiro de Souza T. et al. Melatonin loaded lecithin-chitosan nanoparticles improved the wound healing in diabetic rats. Int J Biol Macromol 2020; 162: 1465-1475
    CrossrefPubMedGoogle Scholar
  • 25 Khalmuratova R, Kim DW, Jeon SY. Effect of dexamethasone on wound healing of the septal mucosa in the rat. Am J Rhinol Allergy 2011; 25 (03) 112-116
    Google Scholar
  • 26 Khalmuratova R, Jeon SY, Kim DW. et al. Wound healing of nasal mucosa in a rat. Am J Rhinol Allergy 2009; 23 (06) e33-e37
    Google Scholar
  • 27 Çakan D, Uşaklıoğlu S. The effect of locally administered phenytoin on wound healing in an experimental nasal septal perforation animal model. Eur Arch Otorhinolaryngol 2022; 279 (07) 3511-3517
    CrossrefPubMedGoogle Scholar
  • 28 Broughton II G, Janis JE, Attinger CE. The basic science of wound healing. Plast Reconstr Surg 2006; 117 (7, suppl): 12S-34S
    CrossrefPubMedGoogle Scholar
  • 29 Elsherbini AM, Ezzat SK. Effect of melatonin versus injectable platelet rich fibrin on critical wound healing in submandibular salivary glands of diabetic rats. J Oral Biol Craniofac Res 2020; 10 (04) 592-596
    CrossrefPubMedGoogle Scholar
  • 30 Song R, Ren L, Ma H. et al. Melatonin promotes diabetic wound healing in vitro by regulating keratinocyte activity. Am J Transl Res 2016; 8 (11) 4682-4693
    PubMedGoogle Scholar
  • 31 Soriano JL, Calpena AC, Rincón M. et al. Melatonin nanogel promotes skin healing response in burn wounds of rats. Nanomedicine (Lond) 2020; 15 (22) 2133-2147
    PubMedGoogle Scholar
  • 32 Diegelmann RF, Evans MC. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci 2004; 9: 283-289
    CrossrefPubMedGoogle Scholar
  • 33 Rujitanaroj PO, Aid-Launais R, Chew SY, Le Visage C. Polysaccharide electrospun fibers with sulfated poly(fucose) promote endothelial cell migration and VEGF-mediated angiogenesis. Biomater Sci 2014; 2 (06) 843-852
    CrossrefPubMedGoogle Scholar
  • 34 Ganguly K, Sharma AV, Reiter RJ, Swarnakar S. Melatonin promotes angiogenesis during protection and healing of indomethacin-induced gastric ulcer: role of matrix metaloproteinase-2. J Pineal Res 2010; 49 (02) 130-140
    PubMedGoogle Scholar
  • 35 Ramírez-Fernández MP, Calvo-Guirado JL, de-Val JE. et al. Melatonin promotes angiogenesis during repair of bone defects: a radiological and histomorphometric study in rabbit tibiae. Clin Oral Investig 2013; 17 (01) 147-158
    CrossrefPubMedGoogle Scholar
  • 36 Satish L, Kathju S. Cellular and molecular characteristics of scarless versus fibrotic wound healing. Dermatol Res Pract 2010; 2010: 790234
    CrossrefPubMedGoogle Scholar
  • 37 Gómez-Florit M, Ramis JM, Monjo M. Anti-fibrotic and anti-inflammatory properties of melatonin on human gingival fibroblasts in vitro. Biochem Pharmacol 2013; 86 (12) 1784-1790
    CrossrefPubMedGoogle Scholar
  • 38 Reinke JM, Sorg H. Wound repair and regeneration. Eur Surg Res 2012; 49 (01) 35-43
    CrossrefPubMedGoogle Scholar