Abstract
Background Ischemia/reperfusion has been reported to further
damage the intestine reperfusion injury (IRI) and cause multiple distal organ
dysfunction through oxidative stress, inflammation, and apoptosis. Cysteamine is
known to inhibit oxidative stress, inflammatory cytokines and apoptosis. This
experiment was designed to evaluate the role of cysteamine against IRI in
rats
Methods Thirty-two Wistar rat strains were assigned to four groups: sham,
Intestinal-reperfusion injury (IRI), 50 mg/kg and
100 mg/kg cysteamine treatment IRI. A 5 cm segment of
terminal ileum was twisted 360° clockwise along the mesentery for
45 minutes to induce ischemia before detorsion. Tissues were preserved
for biochemical evaluation and histology 4 hours after detorsion.
Activities of GPx, GSH, protein and non-protein thiol,
H2O2, MDA were evaluated. Serum concentration of
nitrite, MPO, ALT, AST TNF-alpha and IL-6 were measured. Caspase 3 and bax were
evaluated by immunohistochemistry. Statistical significance was set as
p<0.05
Results Significant (p<0.05) increase in
H2O2, MDA and nitrite but reduction in GPx, GSH,
protein thiol and non-protein thiol in the IRI rats was reversed by 50 and
100 mg/kg cysteamine. Serum MPO, TNF-α, IL6, AST and ALT
was significantly elevated in IRI while the rats treated with cysteamine showed
a significant decrease (p<0.05) in the activities of these inflammatory
and hepatic injury markers.
Conclusion Cysteamine mitigate IRI by enhancing intracellular antioxidant
defense system, inhibiting inflammatory mediators and intestinal tissue
expression of pro-apoptotic protein.
Key words
Detorsion - cytokines - apoptosis - hepatic injury - terminal ileum - mesenteric artery - cysteamine antioxidants
