Sex-Dependent Differences in Mouse Placental Gene Expression following a Maternal High-Fat Diet

Matthew R. Grace; Neeta L. Vora; Lisa Smeester; Sarah K. Dotters-Katz; Rebecca C. Fry; Victoria Bae-Jump; Kim Boggess

doi:10.1055/a-2008-8286

American Journal of Perinatology, Table of Contents

Am J Perinatol 2024; 41(S 01): e1273-e1280
DOI: 10.1055/a-2008-8286

Original Article

Sex-Dependent Differences in Mouse Placental Gene Expression following a Maternal High-Fat Diet

Matthew R. Grace

¹Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee

,

Neeta L. Vora

²Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, University of North Carolina School of Medicine, Chapel Hill, North Carolina

,

Lisa Smeester

³Institute for Environmental Health Solutions, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina

⁴Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina

,

Sarah K. Dotters-Katz

⁵Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, Duke University, Durham, North Carolina

,

Rebecca C. Fry

³Institute for Environmental Health Solutions, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina

⁴Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina

,

Victoria Bae-Jump

⁶Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of North Carolina School of Medicine, North Carolina

,

Kim Boggess

⁷Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, University of North Carolina School of Medicine, Chapel Hill, North Carolina

› Author Affiliations

Abstract

Objective In utero fetal exposures may have sex-specific placental gene responses. Our objective was to measure sex-based differences in placental gene expression from dams fed high-fat diet (HFD) versus control diet (CD).

Study Design We fed timed pregnant Friend virus B–strain dams either a CD (n = 5) or an HFD (n = 5). We euthanized dams on embryonic day 17.5 to collect placentas. We extracted placental RNA and hybridized it to a customized 96-gene Nanostring panel focusing on angiogenic, inflammatory, and growth genes. We compared normalized gene expression between CD and HFD, stratified by fetal sex, using analysis of variance. Pathway analysis was used to further interpret the genomic data.

Results Pups from HFD-fed dams were heavier than those from CD-fed dams (0.97 ± 0.06 vs. 0.84 ± 0.08 g, p < 0.001). Male pups were heavier than females in the HFD (0.99 ± 0.05 vs. 0.94 ± 0.06 g, p = 0.004) but not CD (0.87 ± 0.08 vs. 0.83 ± 0.07 g, p = 0.10) group. No sex-based differences in placental gene expression in CD-fed dams were observed. Among HFD-fed dams, placentas from female pups exhibited upregulation of 15 genes (q = 0.01). Network analyses identified a cluster of genes involved in carbohydrate metabolism, cellular function and maintenance, and endocrine system development and function (p = 1 × 10⁻²³). The observed female-specific increased gene expression following in utero HFD exposure was predicted to be regulated by insulin (p = 5.79 × 10⁻¹³).

Conclusion In female compared with male pups, in utero exposure to HFD upregulated placental gene expression in 15 genes predicted to be regulated by insulin. Sex-specific differences in placental expression of these genes should be further investigated.

Key Points

Male pups were heavier than female pups at the time of sacrifice when dams were fed an HFD.
HFD was associated with upregulated gene expression in female placentas.
Female-specific increased gene was predicted to be regulated by insulin.

Keywords

placental gene expression - high-fat diet - sex differences - insulin

Full Text

References

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Supplementary Material

Supplementary Material