Immunologische Tracerentwicklung mit Nanobodies

Zusammenfassung

Nanobodies sind die antigenbindenden Fragmente eines speziellen Antikörperformats, welches zum Beispiel in Kameliden vorkommt. Die Größe, Struktur und Beschaffenheit von Nanobodies bringen viele Vorteile mit sich, wodurch sie als exzellente Vehikel für die Entwicklung von Radiotracern in der nuklearmedizinischen Bildgebung und Therapie von großem Wert sind. Zu diesem Zweck werden die entsprechenden Radiohalogene oder Radiometalle auf unterschiedliche Art und Weise selektiv oder unselektiv an der Peptidkette des Nanobody’s angebracht. Die indirekte Radiohalogenierung mittels prosthetischer Gruppen und die Postmarkierung von bereits eingebrachten Chelatoren mit Radiometallen sind die bevorzugten Markierungsstrategien. Am häufigsten erfolgt die Tracerentwicklung über den His₆-Tag mit ^99mTc-Tricarbonyl. Präklinisch und auch zum Teil klinisch konnte die Eignung von verschiedenen nanobodybasierten Tracern bereits gezeigt werden.

Abstract

Nanobodies are the antigen-binding fragments of a special antibody format, which for example can be found in camelids. The size, structure and nature of nanobodies implies many advantages, by which they are of great value as excellent vehicles for the development of radiotracers in nuclear medical imaging and therapy. For this purpose, the appropriate radiohalogens or radiometals are attached in different ways selectively or unselectively to the peptide chain of the nanobody. The indirect radiohalogenation by means of prosthetic groups and the post-labeling of already introduced chelators with radiometals are the preferred labeling strategies. Most commonly, the tracer development takes place through the His₆-tag with ^99mTc-tricarbonyl. Preclinically and also in parts clinically, the suitability of different nanobody-based tracers could already be demonstrated.

Schlüsselwörter

Nanobody - Radiohalogene - Radiometalle - Bildgebung - Therapie

Keywords

Nanobody - radiohalogens - radiometals - imaging - therapy

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