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Int J Sports Med
DOI: 10.1055/a-2361-2840
Training & Testing

Living High-Training Low on Mice Bone Parameters Analyzed through Complex Network Approach

Wladimir Rafael Beck 
1   Physiological Sciences, Federal University of Sao Carlos, São Carlos, Brazil
,
Pedro Paulo Menezes Scariot
2   School of Applied Sciences, University of Campinas, Limeira, Brazil
,
Marcelo Papoti
3   School of Physical Education and Sport of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil
,
Taciane Maria Melges Pejon 
1   Physiological Sciences, Federal University of Sao Carlos, São Carlos, Brazil
,
Emanuel Elias Camolese Polisel
2   School of Applied Sciences, University of Campinas, Limeira, Brazil
,
Fúlvia Barros Manchado-Gobatto 
2   School of Applied Sciences, University of Campinas, Limeira, Brazil
,
Claudio Alexandre Gobatto
2   School of Applied Sciences, University of Campinas, Limeira, Brazil
› Author Affiliations Funding Information Coordenação de Aperfeiçoamento de Pessoal de Nível Superior — http://dx.doi.org/10.13039/501100002322; 001 Fundação de Amparo à Pesquisa do Estado de São Paulo — http://dx.doi.org/10.13039/501100001807; 2015/00272–6 2015/01362–9 2017/10201–4 2019/05115–7 2019/08148–3 2019/20930–9 2021/03951–2 Conselho Nacional de Desenvolvimento Científico e Tecnológico — http://dx.doi.org/10.13039/501100003593; Process no 307718/2018–2 Process no 309832/2021–7 Process no 409521/2021–3
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Abstract

The aim of this study was to investigate the effect of 8 weeks of hypoxic exposition and physical training on healthy mice femur outcomes analyzed through conventional statistic and complex networks. The mice were divided into four groups, subjected to physical training (T; 40 min per day at 80% of critical velocity intensity) or not (N), exposed to hypoxic environment (“Living High-Training Low” model – LHTL; 18 h per day, FIO2=19.5%; Hyp) or not (Nor). The complex network analysis performed interactions among parameters using values of critical “r” of 0.5 by Pearson correlations to edges construction, with Fruchterman-Reingold layout adopted for graph visualization. Pondered Degree, Betweenness, and Eigenvector metrics were chosen as centrality metrics. Two-way ANOVA, t-test and Pearson correlation were used with P<0.05. Femur phosphorus of T-Hyp was higher than all other groups (P<0.05) and correlated with bone density (r=0.65; P=0.042), bone mineral density (r=0.67; P=0.034) and% of mineral material (r=0.66, P=0.038). Overall, the complex network demonstrated improvements in bone volume, % of mineral material, bone density, and bone mineral density for T-Hyp over other groups. Association of physical training and hypoxia improved bone quality for healthy mice.

Keywords

hypoxic environment - bone mineral density - critical velocity - physical training - animal model - femur


Publication History

Received: 07 March 2024

Accepted: 01 July 2024

Article published online:
13 November 2024

© 2024. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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