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Horm Metab Res 2008; 40(11): 760-766
DOI: 10.1055/s-0028-1082041
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Variety of Angiotensin Receptors in 3T3-L1 Preadipose Cells and Differentiated Adipocytes

F. Weiland 1 , E. J. Verspohl 1
  • 1Department of Pharmacology, Institute of Pharmaceutical and Medicinal Chemistry, University of Münster, Münster, Germany
Further Information

Publication History

received 20.02.2008

accepted 15.04.2008

Publication Date:
18 August 2008 (online)

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Abstract

A local paracrine acting angiotensin (ANG) system of preadipocytes and mature adipocytes is involved in metabolic effects and tissue differentiation. The present study reports on the investigation of binding affinities for various angiotensin receptors including their relevance in 3T3-L1 adipocytes and preadipocytes and 3T3-442A preadipocytes. Competitive binding studies using both 125I-ANG II and its more stable analogue 125I-SARILE for investigating AT1/AT2 binding sites in 3T3-L1 preadipocytes reveal a biphasic competition curve with KDs at a low and high nanomolar range. By using the AT2 receptor selective ligand 125I-CGP4112A the presence of high affinity AT2 binding sites in preadipocytes was observed. High nonspecific binding and a low receptor number is characteristic for all these experiments. An AT4 binding site (binding site for ANG IV) exists in 3T3-L1 and F442A preadipocytes and adipocytes with a high nanomolar KD. This low binding affinity was confirmed by a biological assay, the IRAP assay (=insulin regulated aminopeptidase assay). IRAP is associated with the AT4 receptor, which is a binding site at the luminal part of membrane bound IRAP. The curves for competition binding and for inhibition of IRAP activity are superimposable with respect to angiotensin IV. In conclusion, AT1 and AT2 binding sites are present in preadipocytes. AT2 receptor binding affinities are shown in preadipocytes for the first time. The description of a non-AT1/AT2 binding site with low affinity remains speculative albeit of high interest because antidiabetic and obesity related effects of angiotensin peptides and sartanes as antagonists are observed at these high concentrations. Local concentrations of ANG II and their degradation products may be extremely high. The low amounts of AT1 and AT2 binding sites emphasize the relevance of other binding sites in adipose tissue development and metabolic effects. The AT4 binding site seems to be one of the predominant receptors in adipose cells. Other degraded, but still bioactive peptides like ANG III, IV and ANG(1–7), activating receptors not influenced by ANG II, could be of importance.

Key words

adipocytes - angiotensin receptors - angiotensin IV - IRAP

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Correspondence

E. J. Verspohl

Department of Pharmacology

Institute of Pharmaceutical and Medicinal Chemistry

Hittorfstr. 58–62

48149 Münster

Germany

Phone: +49/251/833 33 39

Fax: +49/251/833 21 44

Email: verspoh@uni-muenster.de