Planta Med 2009; 75(3): 222-226
DOI: 10.1055/s-0028-1088376
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Enhancement of the Fungicidal Activity of Amphotericin B by Allicin: Effects on Intracellular Ergosterol Trafficking

Akira Ogita1 , Ken-ichi Fujita2 , Toshio Tanaka2
  • 1Research Center for Urban Health and Sports, Osaka City University, Osaka, Japan
  • 2Graduate School of Science, Osaka City University, Osaka, Japan
Further Information

Publication History

Received: August 25, 2008 Revised: October 16, 2008

Accepted: October 23, 2008

Publication Date:
03 December 2008 (online)

Abstract

In Saccharomyces cerevisiae, ergosterol was visible in the plasma membrane of untreated cells and was enriched in the vacuole membrane in response to amphotericin B (AmB) treatment at a non-lethal concentration. The simultaneous addition of allicin was inhibitory to AmB-induced ergosterol enrichment in the vacuole membrane, resulting in increased sensitivity of the organelles to the disruptive action of AmB. Allicin was also inhibitory to ergosterol enrichment in the vacuole membrane that was achieved by its external addition to cells. The combined fungicidal activity of AmB and allicin was suppressed together with suppression of vacuole membrane damage in cells where ergosterol had been fully enriched in the vacuole membrane. AmB caused direct disruptive damage of the isolated vacuoles, but the antibiotic was apparently less effective in disrupting the organelles that were isolated after ergosterol enrichment. These findings suggest that allicin enhances AmB-induced vacuole membrane damage by inhibiting ergosterol trafficking from the plasma membrane to the vacuole membrane.

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Prof. Dr. Toshio Tanaka

Graduate School of Science

Osaka City University

3-3-138 Sugimoto

Sumiyoshi-ku

Osaka 558–8585

Japan

Phone: +81-6-6605-3163

Fax: +81-6-6605-3164

Email: tanakato@sci.osaka-cu.ac.jp