Lanostanes from Phellinus igniarius and Their iNOS Inhibitory Activities

Abstract

Four new lanostanol-type triterpenoids, igniarens A – D (1–4), were isolated from the fruit body of Phellinus igniarius together with two known triterpenoids, and two known ergostanes. These four new compounds were identified by spectroscopic analysis as 22R-hydroxy-24-methylene-29-norlanost-7, 9(11)-dien-3-one (1), 3α,22R-dihydroxy-24-methylene-29-norlanost-7, 9(11)-diene (2), 3α,22R-dihydroxy-24-methylene-29-norlanost-8-ene (3), and 3α,22R-dihydroxy-24-methylenelanost-8-ene (4). Their effects on NO production in lipopolysaccharide (LPS)-activated macrophages were assessed. Compounds 1–8 inhibited NO production in activated RAW 264.7 cells to various degrees. The most potent compound 5α,8α-epidioxy-22E-ergosta-6,22-dien-3β‐ol (7) significantly inhibited LPS-induced NO production in a concentration-dependent manner without affecting the cellular viability, with an IC₅₀ of 37.57 ± 1.38 µM.

Key words

Phellinus igniarius - Hymenochaetaceae - triterpenoids - igniarens - inflammation - macrophages - nitric oxide

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