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Planta Med 2009; 75(15): 1602-1607
DOI: 10.1055/s-0029-1185813
Natural Product Chemistry
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Lanostanes from Phellinus igniarius and Their iNOS Inhibitory Activities

Guei-Jane Wang1 , Tung-Hu Tsai2 , Tun-Tschu Chang3 , Cheng-Jen Chou1 , Lie-Chwen Lin1 , 2 , 4
  • 1National Research Institute of Chinese Medicine, Taipei, Taiwan
  • 2Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan
  • 3Division of Forest Protection, Taiwan Forestry Research Institute, Taipei, Taiwan
  • 4Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan
Further Information

Publication History

received February 28, 2009 revised May 16, 2009

accepted May 20, 2009

Publication Date:
25 June 2009 (online)

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Abstract

Four new lanostanol-type triterpenoids, igniarens A – D (1–4), were isolated from the fruit body of Phellinus igniarius together with two known triterpenoids, and two known ergostanes. These four new compounds were identified by spectroscopic analysis as 22R-hydroxy-24-methylene-29-norlanost-7, 9(11)-dien-3-one (1), 3α,22R-dihydroxy-24-methylene-29-norlanost-7, 9(11)-diene (2), 3α,22R-dihydroxy-24-methylene-29-norlanost-8-ene (3), and 3α,22R-dihydroxy-24-methylenelanost-8-ene (4). Their effects on NO production in lipopolysaccharide (LPS)-activated macrophages were assessed. Compounds 1–8 inhibited NO production in activated RAW 264.7 cells to various degrees. The most potent compound 5α,8α-epidioxy-22E-ergosta-6,22-dien-3β‐ol (7) significantly inhibited LPS-induced NO production in a concentration-dependent manner without affecting the cellular viability, with an IC50 of 37.57 ± 1.38 µM.

Key words

Phellinus igniarius - Hymenochaetaceae - triterpenoids - igniarens - inflammation - macrophages - nitric oxide

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Prof. Dr. Lie-Chwen Lin

National Research Institute of Chinese Medicine

Li-Nong

Taipei 112

Taiwan

Republic of China

Phone: + 88 62 28 20 19 99 ext. 71 01

Fax: + 88 62 28 26 42 76

Email: lclin@nricm.edu.tw