Abstract
Salvia species have an extensive distribution and a long history of medical use in China. In this study, chemical differences between different Salvia species were thoroughly investigated, and 86 constituents were characterized through a sensitive HPLC-DAD-ESI‐MSn method. In total, 19 Salvia species were screened. Our study suggested that Ser. Brachylomae , Ser. Digitaloidites , Ser. Castaneae , and Ser. Miltiorrhizae were the sibling series (Ser. is short for series) and that Ser. Brachylomae , Ser. Maximowicziana , and Ser. Campanulatae were unique with regard to their constituents in addition to having componential affinity. This study offered a simple chemotaxonomy approach and a reasonable bioactivity interpretation of Salvia species.
Key words
chemotaxonomy - Labiatae - LC/MS -
Salvia species - similarity
References
1 Flora of China Editorial Committee .Flora of China, Volume 66. Beijing; Science Press 1977: 70-80
2 China Pharmacopoeia Committee .Pharmacopoeia of P. R. China, Part I. Beijing; Chemical Industry Press 2005: 52-53
3
Luo H Y, Cheng T J.
Comparison of energy metabolism on brain lacking oxygen between Salvia miltiorrhiza Bunge injection and Salvia przewalskii Maxim injection in mice.
Chin J Clin Pharm Ther.
2004;
9
174-176
4
Qu Y W, Li Z, Wang S Y.
Salvia species: a review of phytochemistry and biological activities.
J Hebei Med Uni.
2005;
26
701-702
5
Kasimu R, Tanaka K, Tezuka Y, Gong Z N, Li J X, Basnet P, Namba T, Kadota S.
Comparative study of seventeen Salvia plants: aldose reductase inhibitory activity of water and MeOH extracts and liquid chromatography-mass spectrometry (LC‐MS) analysis of water extracts.
Chem Pharm Bull.
1998;
46
500-504
6
Papp I, Apáti P, Andrasek V, Blázovics A, Kursinszki L, Kite G C, Houghton P J, Kéry Á.
LC‐MS analysis of antioxidant plant phenoloids.
Chromatographia.
2004;
60 (Suppl. 1)
S93-S100
7
Liu A H, Guo H, Ye M, Lin Y H, Sun J H, Xu M, Guo D A.
Detection, characterization and identification of phenolic acids in Danshen using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry.
J Chromatogr A.
2007;
1161
170-182
8
Gu W H.
Review: phytochemistry of Salvia species.
Chin Trad Herb Drugs.
1981;
12
41-48
9
Sun J H, Yang M, Han J, Wang B R, Ma X C, Xu M, Liu P, Guo D A.
Profiling the metabolic difference of seven tanshinones using high-performance liquid chromatography/multi-stage mass spectrometry with data-dependent acquisition.
Rapid Commun Mass Spectrom.
2007;
21
2211-2226
10
Yang M, Liu A H, Guan S H, Sun J H, Xu M, Guo D A.
Characterization of tanshinones in the roots of Salvia miltiorrhiza (Dan-shen) by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry.
Rapid Commun Mass Spectrom.
2006;
20
1266-1280
11
Liu A H, Lin Y H, Yang M, Guo H, Guan S H, Sun J H, Guo D A.
Development of the fingerprints for the quality of the roots of Salvia miltiorrhiza and its related preparations by HPLC‐DAD and LC‐MSn .
J Chromatogr B.
2007;
846
32-41
12
Schwaiger S, Seger C, Wiesbauer B, Schneider P, Ellmerer E P, Sturm S, Stuppner H.
Development of an HPLC‐PAD‐MS assay for the identification and quantification of major phenolic edelweiss (Leontopodium alpium Cass.) constituents.
Phytochem Anal.
2006;
17
291-298
13
Ganzera M, Pöcher A, Stuppner H.
Differentiation of Cirsium japonicum and C. setosum by TLC and HPLC‐MS.
Phytochem Anal.
2005;
16
205-209
14
Saracoglu I, Varel M, Harput U S, Nagatsu A.
Acylated flavonoids and phenol glycosides from Veronica thymoides subsp. Pseudocinerea .
Phytochemistry.
2004;
65
2379-2385
15
Simões-Pires C A, Queiroz E F, Henriques A T, Hostettmann K.
Isolation and on-line identification of antioxidant compounds from three Baccharis species by HPLC‐UV‐MS/MS with post-column derivatisation.
Phytochem Anal.
2005;
16
307-314
16
Schmidt C O, Krammer G E, Weber B, Stoeckigt D, Brennecke S, Kindel G, Bertram H J.
Powerful analytical tools for citrus characterization: LC/MS and LC/NMR characterization polymethoxylated flavones.
ACS Symposium Series.
2006;
936
70-79
17
Zhao D X, Fu C X, Chen Y Q, Ma F S.
Transformation of Saussurea medusa for hairy roots and jaceosidin production.
Plant Cell Rep.
2004;
23
468-474
18
Weber M, Knoy C, Kindscher K, Brown R CD, Niemann S, Chapman J.
Identification of medicinally active compounds in prairie plants by HPLC coupled to electron impact-mass spectrometry.
Am Lab.
2007;
39
9-11
19
Almela L, Sánchez-Muñoz B, Fernández-López J A, Roca M J, Rabe V.
Liquid chromatographic-mass spectrometric analysis of phenolics and free radical scavenging activity of rosemary extract from different raw material.
J Chromatogr A.
2006;
1120
221-229
20
Modugno F, Ribechini E, Colombini M P.
Chemical study of triterpenoid resinous materials in archaeological findings by means of direct exposure electron ionisation mass spectrometry and gas chromatography/mass spectrometry.
Rapid Commun Mass Spectrom.
2006;
20
1787-1800
21
Kuźma Ł, Skrzypek Z, Wysokińska H.
Diterpenoids and triterpenoids in hairy roots of Salvia sclarea .
Plant Cell Tissue Org Cult.
2006;
84
171-179
22
Cai J B, Lin P, Zhu X L, Su Q D.
Comparative analysis of clary sage (S. sclarea L.) oil volatiles by GC-FTIR and GC‐MS.
Food Chem.
2006;
99
401-407
23
Menezes F DS, Kaplan M AC.
In-mixture analysis of triterpenes from Raphiodonechinus .
Rev Latinoam Quim.
2006;
34
37-41
24
Bentayeb K, Rubio C, Batlle R, Nerín C.
Direct determination of carnosic acid in a new active packaging based on natural extract of rosemary.
Anal Bioanal Chem.
2007;
389
1989-1996
25
Fontana G, Savona G, Rodríguez B, Dersch C M, Rothmanc R B, Prisinzano T E.
Synthetic studies of neoclerodane diterpenoids from Salvia splendens and evaluation of opioid receptor affinity.
Tetrahedron.
2008;
64
10041-10048
26
Xiao X H, Fang Q M, Xia W J, Yin G P, Su Z W, Qiao C Z.
Numerical taxonomy of medicinal Salvia L. and genuineness of Danshen.
J Plant Res Env.
1997;
6
17-21
27
Cao Y H, Wang L C, Yu X J, Ye J N.
Development of the chromatographic fingerprint of herbal preparations Shuang-Huang-Lian oral liquid.
J Pharm Biomed Anal.
2006;
41
845-856
Prof. Dr. De-an Guo Dr. Jian Han
Department of Natural Medicines and the State Key Laboratory of Natural and Biomimetic Drugs Peking University Health Science Center
38 Xueyuan Road
Beijing 100191
People's Republic of China
Telefon: + 86 10 82 80 15 16
Fax: + 86 10 82 80 27 00
eMail: gda@bjmu.edu.cn
eMail: hanj@bjmu.edu.cn