Antibacterial Compounds from Mushrooms II: Lanostane Triterpenoids and an Ergostane Steroid with Activity Against Bacillus cereus Isolated from Fomitopsis pinicola

Abstract

Anti−Bacillus cereus bioassay-guided fractionation of a crude extract of the American mushroom, Fomitopsis pinicola, was performed using thin-layer chromatography, Sephadex LH-20 column chromatography, and preparative-scale HPLC. Five lanostane triterpenoids (1–5) and one ergostane steroid (6) were isolated and identified. Compound 1 is a new lanostane triterpenoid, and its structure was determined using 1D and 2D NMR experiments, HR‐MS, and physical data. Each of the purified compounds (1–6) was tested for antibacterial activity against B. cereus using standard MIC assays. Compounds 1–6 had MIC values of 32, 16, 32, 32, 128, and 64 µg/mL, respectively.

antibacterial activity - Fomitopsis pinicola (Polyporaceae) - lanostane triterpenoid - ergostane steroid - Bacillus cereus

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