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Planta Med 2010; 76(8): 766-772
DOI: 10.1055/s-0029-1240701
Pharmacology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Comparison of the Enteric Mucosal Immunomodulatory Activity of Combinations of Coptis chinensis Franch. Rhizomes and Evodia rutaecarpa (Juss.) Benth. Fruits in Mice with Dextran Sulphate Sodium-Induced Ulcerative Colitis

Yingying Luo1 , Hongyan Zhao2 , Zhenli Liu2 , Dahong Ju2 , Xiaojuan He3 , Cheng Xiao4 , Guoyue Zhong5 , Shilin Chen6 , Dajian Yang6 , Albert Sun-chi Chan6 , Aiping Lu3 , 6 , 7
  • 1Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, P. R. China
  • 2Institute of Basic Theory of TCM, China Academy of Chinese Medical Sciences, Beijing, P. R. China
  • 3Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, P. R. China
  • 4Institute of Clinical Medicine, China-Japan Hospital, Beijing, P. R. China
  • 5Chongqing Academy of Chinese Medical Sciences, Chongqing, P. R. China
  • 6State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, Guangdong, P. R. China
  • 7E-Institute of Shanghai Municipal Education Commission, Shanghai TCM University, Shanghai, P. R. China
Weitere Informationen

Publikationsverlauf

received May 10, 2009 revised Nov. 19, 2009

accepted Nov. 23, 2009

Publikationsdatum:
23. Dezember 2009 (online)

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Abstract

Combinations of crude rhizomes of Coptis chinensis Franch., Ranunculaceae, and fruits of Evodia rutaecarpa (Juss.) Benth., Rutaceae, at a ratio of 6 : 1 (formula A) and 1 : 6 (formula B) were extracted with boiling water, and their modulatory activity on enteric mucosal immune responses in mice with dextran sulphate sodium-induced ulcerative colitis was investigated. The results showed that both formulas could reduce the severity of inflammation in the colon. Formula A at a low dose can decrease myeloperoxidase (MPO) activity, and formula B was inactive. Both formulas did not affect the percentages of CD4+ and CD8+ T cells in the periphery, but they evoked an increase of CD8+ T cells among the enteric intraepithelial lymphocytes. Formula B at a low dose could increase both CD4+ and CD8+ cells, and formula A at a high dose could only increase CD8+ T cells among the Peyer's patch lymphocytes (p < 0.05). Both formulas did not affect the percentages of CD4+ and CD8+ T cells among the lamina propria lymphocytes, but decreased the serum concentration of IL-1β (p < 0.05 at a low dose of formula A) and enhanced the level of IL-10 in serum (p < 0.05 at a low dose formula B). We conclude that both formulas have a similar modulating effect on enteric mucosal immune responses, the major difference being that formula A could decrease the level of IL-1 β, while formula B could increase the IL-10 dose in serum.

Key words

Ranunculaceae - Coptis chinensis Franch. - Rutaceae - Evodia rutaecarpa (Juss.) Benth. - ulcerative colitis - mice

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Institute of Basic Research in Clinical Medicine
China Academy of Chinese Medical Sciences

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